Changes in the balance between Treg and Th17 cells in patients with chronic hepatitis B |
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Authors: | Zhi-Jun Su Xue-Ping YuRu-Yi Guo De-Song MingLv-Ye Huang Mi-Long SuYong Deng Zhen-Zhong Lin |
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Affiliation: | Department of Infectious Diseases, First Hospital of Quanzhou, Fujian Medical University, Quanzhou 362000, China |
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Abstract: | The purpose of this study was to explore the role of Treg cells, Th17 cells and cytokines associated with Treg/Th17 differentiation in the occurrence, development and outcome of chronic hepatitis B (CHB). To do so, we detected populations of Treg and Th17 cells and their associated cytokines in the peripheral blood of CHB patients. The populations of Treg cells (CD4+CD25highCD127low T cells) and Th17 cells (CD3+CD8-IL-17+ T cells) were analyzed in 46 patients with low to moderate chronic hepatitis B (CHB-LM), 24 patients with severe chronic hepatitis B (CHB-S) and 20 healthy controls (HC) using flow cytometry. The levels of cytokines associated with Treg/Th17 differentiation, including IL-10, TGF-β1, IL-17 and IL-23, were measured by enzyme-linked immunosorbent assay (ELISA). Our study showed that the imbalance of Treg and Th17 cells might play an important role in the occurrence, development and outcome of CHB. |
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Keywords: | CHB, Chronic hepatitis B HBV, Hepatitis B virus PD-1, Procedural death factor-1 Treg, T Th17, T helper 17 NK, Natural killer DC, Dendritic cells IL-17, Interleukin-17 TGF-β1, Transforming growth factor-β1 CHB-LM, Low to moderate chronic hepatitis B CHB-S, Severe chronic hepatitis B HBsAg, Hepatitis B surface ALT, Alanine aminotransferase TBil, Total bilirubin HAV, Hepatitis A virus HCV, Hepatitis C virus HDV, Hepatitis D virus HC, Healthy controls PBMCs, Peripheral blood mononuclear cells PMA, Phorbol 12-myristate-13-acetate Ion, Ionomycin Mon, Monensin AST, Aspartate aminotransferase DBil, Direct bilirubin HBeAg, Hepatitis B e Antigen ETV, Entecavir G-CSF, Granulocyte colony-stimulating factor NF-kappa B, Nuclear factor kappa B ICAM-1, Intercellular adhesion molecule-1 |
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