Probe ligation and real-time detection of KPC,OXA-48, VIM,IMP, and NDM carbapenemase genes |
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| Authors: | G. Cuzon T. Naas P. Bogaerts Y. Glupczynski P. Nordmann |
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| Affiliation: | 1. Service de Bactériologie-Virologie, INSERM U914: Emerging Resistance to Antibiotics, Hôpital de Bicêtre, Assistance Publique-Hôpitaux de Paris, Faculté de Médecine Paris-Sud, France;2. Laboratoire de Bactériologie, Cliniques Universitaires UCL de Mont-Godinne-Dinant, Yvoir, Belgique |
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| Abstract: | The Check-MDR Carba test (Check-Points, Wageningen, Netherlands), which is based on specific molecular recognition of blaNDM, blaKPC, blaOXA-48, blaVIM, and blaIMP genes by DNA probe ligation and real-time PCR detection, was evaluated on 183 well-characterized Gram-negative rods. Representatives of the 5 gene families were accurately identified (specificities and sensitivities of 100%) within 4.5 hours. This test may be helpful to differentiate carbapenem resistance mediated by carbapenemases from those involving other mechanisms. |
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| Keywords: | Detection Carbapenem resistance Real-time PCR |
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