Rapid PCR amplification protocols decrease the turn-around time for detection of antibiotic resistance genes in Gram-negative pathogens |
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| Authors: | Chelsie N. Geyer Nancy D. Hanson |
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| Affiliation: | Creighton University School of Medicine, Department of Medical Microbiology and Immunology, Center for Research in Anti-Infectives and Biotechnology (C.R.A.B), 2500 California Plaza, Omaha, NE 68178, USA |
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| Abstract: | A previously designed end-point multiplex PCR assay and singleplex assays used to detect β-lactamase genes were evaluated using rapid PCR amplification methodology. Amplification times were 16–18 minutes with an overall detection time of 1.5 hours. Rapid PCR amplifications could decrease the time required to identify resistance mechanisms in Gram-negative organisms. |
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| Keywords: | Diagnostic PCR Beta-lactamase genes Rapid amplification |
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