Standardization of multilocus sequence typing scheme for Mycobacterium abscessus and Mycobacterium massiliense |
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| Authors: | Song Yee Kim Young Ae Kang Il Kwon Bae Jae-Joon Yim Moo Suk Park Young Sam Kim Se Kyu Kim Joon Chang Seok Hoon Jeong |
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| Institution: | 1. Division of Pulmonology, Department of Internal Medicine, Yonsei University College of Medicine, Seoul 120-752, Republic of Korea;2. Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul 135-720, Republic of Korea;3. Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine and Lung Institute, Seoul National University College of Medicine, Seoul 110-744, Republic of Korea |
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| Abstract: | This study aims to develop a multilocus sequence typing (MLST) scheme for Mycobacterium abscessus complex for the typing of stains within each species. A total of 89 clinical isolates of M. abscessus complex from 71 patients of 2 tertiary care hospitals in South Korea were included. Forty-two isolates were identified as M. abscessus, and 29, as Mycobacterium massiliense through sequencing of 8 housekeeping genes and rpoB. The MLST scheme identified 26 different sequence types(STs) and 13 different clonal complexes (CCs) in M. abscessus and 12 different STs and 6 different CCs in M. massiliense. The MLST data showed high concordance with the XbaI-macrorestriction patterns of pulsed-field gel electrophoresis in the duplicated isolates. Our MLST schemes could identify different strains of M. abscessus and M. massiliense, and the schemes also showed a reliable reproducibility. Therefore, our MLST schemes may be useful in studying the epidemiology of M. abscessus and M. massiliense infections. |
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| Keywords: | Mycobacterium abscessus Mycobacterium massiliense Multilocus sequence analysis rpoB Multilocus sequence typing |
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