Modification of the Congo red agar method to detect biofilm production by Staphylococcus epidermidis |
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| Authors: | Thaís Dias Lemos Kaiser Eliezer Menezes Pereira Kátia Regina Netto dos Santos Ethel Leonor Noia Maciel Ricardo Pinto Schuenck Ana Paula Ferreira Nunes |
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| Institution: | 1. Laboratório de Resistência Bacteriana, RESBAC, Departamento de Patologia e Programa de Pós-Graduação em Doenças Infecciosas, Universidade Federal do Espírito Santo, Brazil;2. Laboratório de Microbiologia Aplicada e Processos Fermentativos Prof. Denise Bello–LaMProF, Instituto Federal de Educação, Ciência e Tecnologia do Rio de Janeiro, Campus Rio de Janeiro, Rio de Janeiro, Brazil;3. Laboratório de Infecções Hospitalares, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil;4. Departamento de Enfermagem e Programa de Pós-Graduação em Doenças Infecciosas, Universidade Federal do Espírito Santo, Brazil |
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| Abstract: | Staphylococcus epidermidis in immunocompromised patients can cause bacteremia related to the use of catheter due to biofilm production. There are different phenotypic methods to detect biofilm formation. One method is based on culture in brain heart infusion agar (BHIA) containing sucrose and red Congo dye (original Congo red agar). Our group created a new CRA formula and we have confirmed its capacity to detect biofilm production in 210 S. epidermidis strains, including 76 (36.2%) icaAB gene–positive strains. Other parameters were also evaluated. The new CRA formula that gave the best results was BHIA with sucrose (5%), Congo red (0.08%), NaCl (1.5%), glucose (2%), and vancomycin (0.5 mg/mL) (vancomycin-modified CRA—CRAmod). The CRAmod plus vancomycin may be a promising tool and can help to determine the real participation of S. epidermidis in the infectious process. |
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| Keywords: | S epidermidis Biofilm Congo red agar Vancomycin icaAB gene |
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