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A simple technique for evaluation of methods of cell separation |
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| Authors: | H Zola R Krishnan J Bradley |
| Institution: | Department of Clinical Immunology, Flinders Medical Centre, Bedford Park 5042, South Australia, Australia |
| Abstract: | A simple method is described for labelling cells with fluorescein and using them in artificial mixtures to assess cell separation procedures. The method facilitates the examination of the variables in a separation procedure. It is thus possible to tailor a separation procedure (for example panning with monoclonal antibody) to suit the specific requirements of the experiment. |
| Keywords: | cell separation panning monoclonal antibody flow cytometry FCS foetal calf serum FDA fluorescein diacetate FITC fluorescein isothiocyanate PBL peripheral blood lymphocytes PBS phosphate-buffered saline |
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