HBV相关肝癌组织中TAP1及TNF—a的表达及意义

目的探讨TAP1、TNF-a表达与HBV相关肝细胞癌发生发展的关系。方法采用免疫组织化学技术EnVision方法检测35例HBV相关肝癌组织和10例正常肝组织TAP1、TNF-a的表达，并分析其表达与肝癌发生，及与肝癌病理分级的关系。结果正常肝组织TAP1及TNF-d表达阴性，TAP1在HBV相关肝癌组织表达阳性率为91．4％，与正常肝组织表达比较差异有统计学意义（P〈0．01），TNF-a在HBV相关肝癌组织表达阳性率为60％，与正常肝组织表达相比较差异有统计学意义（P〈0．05），并且分化较好的肝癌组织TNF-a阳性表达率显著高于分化较差者（P〈0．05）。结论HBV相关肝癌组织TAP1相关的MHC-I类分子限制的抗原递呈途径可能正常；HBV相关肝癌组织TNF-a表达量与肝癌患病及组织分化程度相关。

Expression of TAP1 and TNF-ain HBV-related hepatocellular carcinoma and its clinical significance

Objective To explore the association of expression of transporter associated with antigen processing（TAP1 ）, tumor necrosis factor-alpha （TNF-ot） with occurrence and development of HBV-related hepatocellular carcinoma（HCC）. Methods The expression of TAP1 and TNF-a in 35 liver carcinoma tissues, and 10 normal liver tissues were detected with immunohistochemical EnVision method,The expression and clinical significance of TAP1, TNF-a in HBV-related HCC were analyzed. Furthermore, the association of respective expression quantity with pathological typing of HCC was analyzed. Results The expression of TAP1 ,TNF-a was negative in hepatocytes of normal liver tissues. 91.4% of HCC tissues expressed TAP1. There was significantly statistical difference in expression of TAP1 between HCC tissues and the normal hepatic tissues（P 〈0.05） ;60% of HCC tissues expressed TNF-a. There was statistical difference in expression of TNF-a between HCC and the normal hepatic tissues （ P 〈 0. 05 ）, and the positive expression rate of TNF-a in well-differenciated HCC tissues was significantly higher than that in poorly-differenciated ones （P 〈 0. 05 ）. Conclusion TAPl-related MHC- I restricted antigen processing pathway was possibly normal in HBV-related HCC. The expression quantity of TNF-a was associated with the occurrence and degree of pathological differentiation of HBV-related HCC.