Addition of zinc methacrylate in dental polymers: MMP-2 inhibition and ultimate tensile strength evaluation |
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Authors: | Sandrina Henn Rodrigo Varella de Carvalho Fabrício Aulo Ogliari Ana Paula de Souza Sergio Roberto Peres Line Adriana Fernandes da Silva Flávio Fernando Demarco Adriana Etges Evandro Piva |
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Institution: | 1.Operative Dentistry Department, School of Dentistry,Federal University of Pelotas,Pelotas,Brazil;2.Department of Restorative Dentistry, School of Dentistry,University of North Parana,Londrina,Brazil;3.School of Materials Engineering,Federal University of Pelotas,Pelotas,Brazil;4.Morphology Department, Piracicaba Dental School,University of Campinas,Piracicaba,Brazil;5.Oral Pathology Department, School of Dentistry,Federal University of Pelotas,Pelotas,Brazil |
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Abstract: | This study evaluated the effect of zinc methacrylate (ZM) on the inhibition of matrix metalloproteinase 2 (MMP-2) and the
ultimate tensile strength (UTS) of an experimental polymer. Enzymes secreted from mouse gingival tissues were analyzed by
gelatin zymography in buffers containing 5 mM CaCl2 (Tris–CaCl2) in 50 mM Tris–HCl buffer with various concentrations of ZM (0.5, 1, 2, 4, 8, and 16 mM). The matrix metalloproteinases present
in the conditioned media were characterized by immunoprecipitation. The polymer UTS evaluation was performed in eight groups
with various concentrations of ZM (0, 0.5, 1, 2.5, 5, 10, 20, and 30 wt.%), in a mechanical testing machine. MMP-2 (62 kDa)
was detected in the zymographic assays and inhibited by ZM in all tested concentrations. UTS data were submitted to one-way
ANOVA and Tukey's test (α = 0.05), and no significant differences were observed among groups, except in the polymer containing 30% ZM, presenting a
significantly lower value when compared with the control group (p < 0.05). The results suggest that ZM inhibits MMP-2 expression in all concentrations tested, while small concentrations did
not affect the ultimate tensile strength of the polymer. Zinc methacrylate is a metalloproteinase inhibitor that can be copolymerized
with other methacrylate monomers. Yet, the addition of ZM did not affect the resin bond strength. Thus, in vivo tests should
be performed to evaluate the performance of this material. |
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