托瑞米芬协同顺铂对人肺癌细胞株A549的影响

目的：研究托瑞米芬（TOR）对人肺腺癌细胞系A549的毒性作用及其与顺铂（DDP）联用的协同效应,探讨肺癌综合治疗的方向。方法：用MTT显色法检测TOR及与DDP联用后对A549细胞的毒性作用,测定其吸光度（A）值。用流式细胞仪检测细胞DNA含量,Western blot法检测p21蛋白表达。结果：TOR能直接抑制A549细胞的生长,≥5μmol/L的TOR可明显增强DDP的细胞毒性作用。TOR可加强DDP对S期、G2期及M期细胞的作用,且DDP＋TOR后p21蛋白表达增加。结论：≥5μmol/L的TOR与DDP联用对A549细胞具有显著的协同抗肿瘤效应。

Synergistic effect of toremifene and cisplatin on human lung cancer cell line A549

OBJECTIVE: To study the toxic effect of toremifene (TOR) and its synergistic effect with cisplatin (DDP) on human lung adenocarcinoma cell line A549. METHODS: The cytotoxic effects of these agents on human lung cancer cell line A549 were monitored by a tetrazolium-based colorimetric assay (MTT assay). The cell cycle and DNA content were detected by flow cytometer technic. p21 expression level was monitored by Western blot. RESULTS: Toremifene inhibited the growth of A549 cell, with > or = 5 micromol/L significantly enhancing the chemosensitivity of cisplatin. TOR enhanced the antitumor activity of DDP at S, G(2) and M phases of cells. And p21 expression was increased after TOR and DDP had been given. CONCLUSION: Toremifene (> or = 5 micromol/L) combined with cisplatin shows significant synergistic anti-tumor effect on A549 cells.