MTHFR基因C677T多态性与汉族人群抑郁症的关联性

目的 探讨N5,N10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T多态性与汉族人群抑郁症发生的关系.方法 纳入符合美国精神障碍诊断与统计手册第4版(DSM-IV)诊断标准的抑郁症患者402例和正常对照者600名,均为汉族.入组时对抑郁症患者进行汉密尔顿抑郁量表(HAMD)-17评定,采用PCR-RFLP技术检测MTHFR基因C677T的多态性.结果 抑郁症组与对照组基因型(CC、CT、TT)分布频率差异均有统计学意义(P<0.05);其中抑郁症组TT基因型频率显著高于正常对照组,差异有统计学意义(P<0.05).抑郁症组CT和TT基因型患者的HAMD-17评分均显著高于CC型,差异有统计学意义(P<0.05).结论 MTHFR基因C677T多态性与抑郁症关联,且TT基因型与抑郁症的严重程度相关.

Association of MTHFR gene C677T polymorphisms and depression in Han populations

Objective To investigate the relationship between N5, N10-methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms and depression in Han populations. Methods Four hundred and two patients with major depressive disorder diagnosed with Diagnostic and Statistical Manual of Mental Disorders 4th Edition (DSM-IV) (major depressive disorder group) and six hundred healthy controls (control group) were included. All subjects were Han nationality. Patients in major depressive disorder group were assessed with Hamilton Rating Scale for Depression (HAMD)-17, and MTHFR gene C677T polymorphisms were determined with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Results There were significant differences in genotype (CC, CT, TT) frequencies between major depressive disorder group and control group (P<0.05). The genotype frequency of TT in major depressive disorder group was significantly higher than that in control group (P<0.05). The HAMD-17 scores of patients with CT and TT genotypes were significantly higher than that of patients with CC genotype in major depressive disorder group (P<0.05). Conclusion MTHFR gene C677T polymorphisms are associated with depression, and genotype TT is related to the severity of depression.