酶联免疫吸附法检测庚型肝炎病毒抗体

目的 探讨抗庚型肝炎病毒（ＨＧＶ）ＩｇＧ与各种病毒感染，丙氨到转氨酶（ＡＬＴ）及ＨＧＶ ＲＮＡ的相关性。方法 用酶联免疫吸附试验（ＥＬＩＳＡ）法检测了３１５例各种肝炎病毒（Ａ￣Ｅ）感染乾和１１７例健康献血员血清的抗－ＨＧＶ ＩｇＧ，并测定其ＡＬＴ，对抗－ＨＧＶ ＩｇＧ阳性的标本用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）法检测ＨＧＶ ＲＮＡ。结果 献血员抗－ＨＧＶ ＩｇＧ的阳性率为３．４２％（４／１１７

Detection of IgG antibody to hepatitis G virus by ELISA

OBJECTIVE: To study the relation of HGV IgG antibody (anti- HGV IgG) to various viral infections, ALT and HGV RNA. METHODS: To detect anti-HGV IgG from 315 persons infected with various viruses hepatitis (A - E) and 117 healthy blood donors by ELISA. The detection of ALT of these peoples, and detect HGV RNA of the samples of positive anti-HGV IgG by RT-PCR were also carried out. RESULTS: The positive rate of anti-HGV IgG of 117 blood donors was 3.42% (4/117), and that of the people infected by non A- E, HAV, HBV, HCV, HEV, HBV and HCV, HBV and HDV, HBV and HEV were 5.95%(5/84), 0(0/3), 3.75% (3/80), 17. 28%(14/81), 0(0/15), 33.33%(5/15), 3.33%(1/30), 16.66%(1/6), respectively. The relativity of anti-HGV IgG and HGV RNA was 30.30% (10/33), and 33.33% of ALT level in the antibody positive samples were elevated. CONCLUSIONS: HGV may infect people alone, or coinfect with HBV or /and HCV, but is highly relative to HCV, and the coinfection of HGV with HCV may increase the degree of liver damage.