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酶联免疫吸附法检测庚型肝炎病毒抗体
引用本文:郑有为,梁敏文,王景健. 酶联免疫吸附法检测庚型肝炎病毒抗体[J]. 中华实验和临床病毒学杂志, 2000, 14(2): 151-153
作者姓名:郑有为  梁敏文  王景健
作者单位:广东省人民医院检验科
摘    要:目的 探讨抗庚型肝炎病毒(HGV)IgG与各种病毒感染,丙氨到转氨酶(ALT)及HGV RNA的相关性。方法 用酶联免疫吸附试验(ELISA)法检测了315例各种肝炎病毒(A ̄E)感染乾和117例健康献血员血清的抗-HGV IgG,并测定其ALT,对抗-HGV IgG阳性的标本用逆转录-聚合酶链反应(RT-PCR)法检测HGV RNA。结果 献血员抗-HGV IgG的阳性率为3.42%(4/117

关 键 词:病毒抗体 RNA病毒 庚型肝炎 ELISA
修稿时间:1999-06-25

Detection of IgG antibody to hepatitis G virus by ELISA
ZHENG Youwei,LIANG Minwen,WANG Jingjian,et al.. Detection of IgG antibody to hepatitis G virus by ELISA[J]. Chinese journal of experimental and clinical virology, 2000, 14(2): 151-153
Authors:ZHENG Youwei  LIANG Minwen  WANG Jingjian  et al.
Affiliation:Guangdong Provincial People' s Hospital, Guangzhou 510080, China.
Abstract:OBJECTIVE: To study the relation of HGV IgG antibody (anti- HGV IgG) to various viral infections, ALT and HGV RNA. METHODS: To detect anti-HGV IgG from 315 persons infected with various viruses hepatitis (A - E) and 117 healthy blood donors by ELISA. The detection of ALT of these peoples, and detect HGV RNA of the samples of positive anti-HGV IgG by RT-PCR were also carried out. RESULTS: The positive rate of anti-HGV IgG of 117 blood donors was 3.42% (4/117), and that of the people infected by non A- E, HAV, HBV, HCV, HEV, HBV and HCV, HBV and HDV, HBV and HEV were 5.95%(5/84), 0(0/3), 3.75% (3/80), 17. 28%(14/81), 0(0/15), 33.33%(5/15), 3.33%(1/30), 16.66%(1/6), respectively. The relativity of anti-HGV IgG and HGV RNA was 30.30% (10/33), and 33.33% of ALT level in the antibody positive samples were elevated. CONCLUSIONS: HGV may infect people alone, or coinfect with HBV or /and HCV, but is highly relative to HCV, and the coinfection of HGV with HCV may increase the degree of liver damage.
Keywords:Antibody   viral  IgG  RNA   viral  Alanine aminotransferase (ALT)  Engyme linked immunosorbent assay  Hepatitis G virus
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