过敏性哮喘大鼠大脑和肺组织c-fos蛋白的表达

目的：探讨哮喘大鼠肺脏和大脑中c-fos表达的分布及其意义。方法：制作卵蛋白致敏大鼠哮喘模型，采用免疫组织化学ABC方法和图像分析等技术观察Fos蛋白在肺脏和大脑内的分布情况。结果：哮喘组大鼠（10只）肺脏和大脑中c-fos表达与正常对照组大鼠比较，差异有显著性（P＜0．05）；Fos阳性产物在脑内主要集中分布在额顶皮质、边缘前脑（扣带皮质、梨状皮质和中央杏仁核等）、丘脑室旁核、下丘脑室旁核、视上核、下丘脑外侧区、下丘脑室周核、孤束核、最后区和延髓腹外侧区内；小脑内无明显Fos分布密集区。结论：哮喘的发病机制中可能存在神经免疫调节的参与；Fos蛋白可能参与了哮喘的神经免疫调节。

Expression of c-fos protein in brain and lung in ovalbumin sensitized rats

Objective c-fos expression and its distribution in brain and lung of ovalbumin sensitized and challenged rats were investigated.Methods Asthma rat model sensitized and challenged by ova lbumin was established. c-fos expression was detected with immunohistochemical ABC and image analysis methods. Results c-fos positive s taining increased significantly in both brain and lung in asthmatic group as com pared with normal control. (P<0.01). c-fos positive products were mainly concentrated in frontal and parietal cortex, forebrain limbic system (ci ngulated cortex, piriform cortex and central amygdaloid nucleus), paraventricula r thalamic nucleus, hypothalamic paraventricular nucleus, supraoptic nucleus, la teral hypothalamic area, periventricular hypothalamic nucleus, nucleus of solita ry tract, area postrema and ventrolateral medulla. No distinct gathering area of c-fos protein was found in the cerebellum.Conclusion It is suggested that neur oimmunomodulation may play a role in the pathogenesis of asthma and c-fos prote in might be involved in the neuroimmunomodulation.