| 8-硝基白杨素诱导结肠癌HT-29细胞凋亡 |
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| 引用本文： | 伍石华,艾小红,向红琳,岳军,曹建国. 8-硝基白杨素诱导结肠癌HT-29细胞凋亡[J]. 湖南师范大学学报(医学版), 2008, 5(3) |
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| 作者姓名： | 伍石华 艾小红 向红琳 岳军 曹建国 |
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| 作者单位： | 邵阳医学高等专科学校病理教研室,湖南,邵阳,422000;南华大学附属一医院肿瘤内科,湖南,衡阳,421001;湖南师范大学医学院,湖南,长沙,410006 |
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| 基金项目： | 湖南省自然科学基金，湖南省卫生厅医药科研项目 |
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| 摘 要： | 目的:观察8-硝基白杨素(NOChR)诱导人结肠癌HT-29细胞凋亡作用。方法:MTT比色法测定细胞增殖活性;流式细胞分析术检测细胞凋亡率;琼脂糖凝胶电泳观察DNA梯形条带。结果:NOChR抑制HT-29细胞活性,呈浓度依赖性,其IC50值为1.78μM;NOChR具有诱导HT-29细胞凋亡作用;PPARγ特异性阻断剂GW 9662能部分拮抗NOChR诱导HT-29细胞凋亡。结论:NOChR诱导HT-29细胞凋亡作用与其活化PPARγ相关。
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| 关 键 词： | 结肠癌 白杨素 白杨素衍生物 凋亡 |
Induction of Apoptosis of Human Colon Cancer HT-29 Cells by 5,7-Dihydroxy-8-nitrochrysin |
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| WU Shi-Hua,AI Xiao-Hong,XIANG Hong-Lin,YUE Jun,CAO Jian-Guo. Induction of Apoptosis of Human Colon Cancer HT-29 Cells by 5,7-Dihydroxy-8-nitrochrysin[J]. Journal of Hunan Normal University(Medical Science), 2008, 5(3) |
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| Authors: | WU Shi-Hua AI Xiao-Hong XIANG Hong-Lin YUE Jun CAO Jian-Guo |
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| Affiliation: | 1.Department of Pathology; Shaoyang Medical College; Shaoyang; 422000; China; 2.Department of Oncology; First Affiliated Hospital; Nahua University; Hengyang; 421001; 3.Medical College; Hunan Normal University; Changsha; 410006; China); |
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| Abstract: | Objective To investigate induction of apoptosis of human colon cancer HT-29 cells by 5,7-dihydrox-8-nitrochrysin in vitro.Methods The proliferative activity of HT-29 cells treated with 5,7-dihydrox-8-nitrochrysin was measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-dipheny tertrazolium blue(MTT) colorimetric assay.HT-29 cell apoptotic percentage was detected by flow cytometry with PI staining.DNA ladder bands were observed by DNA agarose gel electrophoresis.Results MTT assay suggested that 5,7-dihydrox-8-nit... |
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| Keywords: | human colon cancer chrysin chrysin derivatives apoptosis |
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