Characterization of the UL4 gene product of herpes simplex virus type 2 |
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Authors: | H Yamada Y-M Jiang S Oshima K Wada F Goshima T Daikoku Y Nishiyama |
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Institution: | (1) Laboratory of Virology, Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya, Japan, JP |
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Abstract: | Summary. We have identified the herpes simplex virus type 2 (HSV-2) UL4 gene product using a rabbit polyclonal antiserum raised against
a recombinant 6xHis-UL4 fusion protein expressed in Escherichia coli. The antiserum reacted specifically with a 27-kDa protein in HSV-2 186-infected cell lysates. The protein was not detectable
in the presence of the viral DNA synthesis inhibitor, suggesting that the UL4 gene was expressed as a γ2 gene. Indirect immunofluorescence studies localized the UL4 protein within the nucleus as discrete punctate forms at late
times postinfection. However, when expressed in the absence of other viral proteins, the UL4 protein was limited to the cytoplasm,
indicating that an interaction with one or more other virus-induced proteins was responsible for the nuclear localization
during infection. Subnuclear fractionation studies showed that the protein was released from the nuclear structure of infected
cells by high salt treatment. Moreover, the UL4 protein was detected in purified virions and light particles.
Received December 24, 1997 Accepted February 4, 1998 |
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