表面等离子共振技术检测宫颈人乳头瘤病毒感染的临床研究

目的 研究表面等离子共振（SPR）技术在临床上检测宫颈人乳头瘤病毒（HPV）感染的情况.方法 运用SPR技术诊断502例宫颈HPV患者宫颈脱落细胞的HPV基因亚型,对比SPR技术和核酸分子快速导流杂交基因芯片（HybriMax）技术检测宫颈各级病变的HPV阳性率情况.结果 SPR技术检测出22种HPV基因亚型,单一感染106例,其中细胞学检查阳性47例（44.34％）;多重感染36例,其中细胞学检查阳性16例（44.44％）,2者间差异无统计学意义（P＞0.05）.106例单一感染患者中高危感染93例,其中细胞学检查阳性45例（48.39％）;中低危感染13例,其中细胞学检查阳性2例（15.38％）,2者间差异有统计学意义（P＜0.05）.SPR技术和HybriMax技术对比结果显示,2者检测宫颈各级病变的HPV阳性率结果间差异无统计学意义（P＞0.05）.结论 SPR技术用于检测HPV感染较为可靠,可和HybriMax技术作为临床检测的主要手段.

SPR-HPV detection of human cervical paplllomavirus infection in clinical research

Objective To study the status of surface plasmon resonance （SPR） detection of cervical human papillomavirus （HPV） infection in clinical practice.Methods 502 cases of cervical HPV in patients with cervical cell＇s genetic subtypes were diagnosed using SPR.Positive rates of HPV lesions of cervical levels detected by SPR and nucleic acid molecules rapid flow-through hybridization gene chip （HybriMax） technology were compared.Results Twenty-two kinds of subtypes were detected by SPR.106 women were found with single infection of HPV,including 47 women （44.34%） with abnormal cytological results,while 36 women were found with multiple infection of HPV,including 16 women （44.44%） with abnormal cytological results,and the difference was not significant （P〉0.05）.Among 106 women with single infection of HPV,93 women were found with high risk HPV infection,including 45 women （48.39%） with abnormal cytological results,while 13 women were found with middle-low risk HPV infection,including 2 women （15.38%） with abnormal cytological results,and the difference was statistically significant （P〈0.005）.Comparison of SPR and HybriMax technology showed that the two test results showed no significant difference （P〉0.05）.Conclusions SPR for detection of HPV infection is more reliable,it can combine HybriMax as the primary means of clinical detection.