Quantification of regional glial fibrillary acidic protein levels in Alzheimer's disease |
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Authors: | Ross G W O'Callaghan J P Sharp D S Petrovitch H Miller D B Abbott R D Nelson J Launer L J Foley D J Burchfiel C M Hardman J White L R |
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Affiliation: | Department of Veterans Affairs, Honolulu, HI, USA. ross@phri.hawaii-health.com |
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Abstract: | OBJECTIVES: Our objectives were to quantify glial fibrillary acidic protein (GFAP) in brains of Alzheimer's disease (AD) cases, and non-AD controls to determine the regions with the most severe gliosis in AD. MATERIAL AND METHODS: In a case control design, we used an enzyme-linked immunosorbent assay (ELISA) to quantify GFAP in frozen brain from four areas of neocortex in 10 AD cases, 10 age-matched controls, and 10 younger controls from the Honolulu-Asia Aging Study autopsy archive. RESULTS: Median age at death was 83.5 years for cases and age-matched controls, and 77 years for younger controls. For the AD cases compared with the age-matched controls, levels of GFAP in occipital (P=0.01), parietal (P=0.028), and temporal lobes (P=0.004) (but not frontal) were significantly higher in the cases. The median GFAP excess in AD cases compared with age matched controls was highest in the temporal lobe. CONCLUSIONS: Regional quantification of GFAP reveals that the glial response is most prominent in the temporal lobe in AD. |
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Keywords: | Gliosis Alzheimer disease dementia glial fibrillary acidic protein |
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