| 大鼠骨髓间充质干细胞与大鼠胰腺岛管上皮细胞间接共培养向胰岛细胞分化的实验研究 |
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| 引用本文： | 王海澜,龙爱梅,马真,李慧丰. 大鼠骨髓间充质干细胞与大鼠胰腺岛管上皮细胞间接共培养向胰岛细胞分化的实验研究[J]. 实用医技杂志, 2010, 17(3): 200-202,F0003 |
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| 作者姓名： | 王海澜 龙爱梅 马真 李慧丰 |
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| 作者单位： | 1. 深圳市龙岗中心医院,518116
2. 深圳市龙岗区慢病院 |
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| 基金项目： | 广东省自然科学基金博士启动项目(8445583547-7001223) |
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| 摘 要： | 目的探讨骨髓间充质干细胞(BMSCs)在适当条件下体外分化胰岛素分泌细胞的可能性。方法分离大鼠BMSCs及大鼠胰腺导管上皮细胞分别培养,在大鼠BMSCs培养过程中加入大鼠胰腺导管上皮细胞培养基中的上清部分,双硫腙(DTZ)染色观察诱导后的细胞着色情况,培养7d后进行了反转录-聚合酶链反应(RT-PCR)检测目的基因的表达情况。结果将BMSCs进行分离和纯化,培养至第4代第6天时细胞形态一致,呈"水草"样排列,大鼠胰腺导管上皮细胞表达CK-19;混合培养基诱导的BMSCsDTZ染色阳性,RT-PCR检测有胰腺-十二指肠同源盒基因(PDX-1)及胰岛素(Ins)表达。结论适当的培养环境下,大鼠BMSCs具有向胰岛素分泌细胞分化的能力。
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| 关 键 词： | 间质干细胞 胰腺导管上皮细胞 胰岛细胞 胰腺-十二指肠同源盒基因 |
Bone marrow mesrnchymal stem cells differentiate into insulin producing cells as co-culturing with pancreatic ductai epithelial cells |
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| WANG Hai-lan,LONG Ai-mei,MA Zhen,LI Hui-feng.Longgang District Center Hospital in Shenzhen City,Guangdong ,China. Bone marrow mesrnchymal stem cells differentiate into insulin producing cells as co-culturing with pancreatic ductai epithelial cells[J]. Journal of Practical Medical Techniques, 2010, 17(3): 200-202,F0003 |
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| Authors: | WANG Hai-lan LONG Ai-mei MA Zhen LI Hui-feng.Longgang District Center Hospital in Shenzhen City Guangdong China |
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| Affiliation: | WANG Hai-lan,LONG Ai-mei,MA Zhen,LI Hui-feng.Longgang District Center Hospital in Shenzhen City,Guangdong 518116,China |
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| Abstract: | Objective To investigate the feasibility of bone marrow mesenchymal stem cells differentiate into insulin producing cells in appropriate vitro environment.Methods Rat bone marrow mesenchymal stem cells and rat pancreatic ductal epithelial cells were isolated and cultured respectively.Add the supernatant medium pancreatic ductal epithelial cells was cultured into the medium The identity of differentiated cells was confirmed by dithizone staining and analysis of the gene expression for endocrine markers(PDX-1... |
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| Keywords: | Mesenchymal stem cells Pancreatic ductal epithelial cell Islet cells Pancreatic duodenal homebox gene |
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