Down‐regulated expression of SATB2 is associated with metastasis and poor prognosis in colorectal cancer |
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Authors: | Shuang Wang Jun Zhou Xiao‐Yan Wang Jun‐Mei Hao Juan‐Zhi Chen Xiang‐Mei Zhang He Jin Li Liu Yan‐Fei Zhang Jinsong Liu Yan‐Qing Ding Jian‐Ming Li |
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Affiliation: | 1. Department of Pathology, School of Basic Medical Sciences, Southern Medical University, Guangzhou 510515, People's Republic of China;2. Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, People's Republic of China;3. Guangdong Provincial Key Laboratory of Molecular Tumor Pathology, Guangzhou 510515, People's Republic of China;4. Department of Pathology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA |
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Abstract: | To identify novel biomarkers of metastasis of colorectal cancer (CRC), we developed an orthotopic implantation model of murine CRC and selected in vivo M5, a subclone of the SW480 CRC cell line with enhanced potential for metastasis to the liver. We compared the differences in the gene expression profiles between M5 and SW480 cells using gene expression profiling. We found that expression of special AT‐rich sequence‐binding protein 2 (SATB2) was down‐regulated in M5 cells. Immunohistochemical analysis of 146 colorectal tumour samples showed that underexpression of SATB2 was strongly correlated with poor prognosis, tumour invasion, lymph node metastasis, distant metastasis, and Dukes' classification for CRC. Univariate and multivariate survival analyses further showed that SATB2 expression was a potential favourable prognostic factor for CRC. These results demonstrated not only that SATB2 is a potential novel prognostic factor for CRC, but also that selection of a highly metastatic clone of SW480 in vivo coupled with gene expression profiling is a powerful approach to identifying prognostic markers for CRC. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd. |
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Keywords: | SATB2 prognosis tumour progression CRC |
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