艾迪粉针剂的抗肿瘤作用

目的通过体内外抗肿瘤实验观察艾迪粉针剂的抗肿瘤作用。方法抗肿瘤体内实验:建立小鼠移植性肉瘤S180和小鼠肝癌H22的实体瘤模型,艾迪粉针剂对小鼠尾静脉给药,连续处理7 d,第8天剥离瘤块称质量,计算抑瘤率,与化疗药合并用药考察减毒增效作用;建立小鼠L1210白血病模型,阳性对照组为顺铂,考察生命延长率。体外实验:常规传代培养人肝癌BEL7402、人宫颈癌HeLa细胞、人黑色素瘤A375 S2细胞、人胃癌SGC 7901和小鼠纤维肉瘤L929细胞,用MTT法检测活细胞数和生长抑制率。结果艾迪粉针剂连续7 d每日1次静脉注射对小鼠肉瘤S180和小鼠肝癌H22具有明显的抑瘤作用,本品与化疗药合并用药有增效作用;艾迪粉针剂体外对多种人肿瘤细胞具有体外抗肿瘤活性,且具有较低的IC50值。结论艾迪粉针剂具有体内和体外抗肿瘤活性。

Antitumor effect of AIDI injectable powder

Objective To observe the antitumor activity of AIDI injectable powder by anti-carcinoma experiments in vivo and in vitro.Methods Tumor-bearing mouse models were set up by transplanting hepatoma(H_(22)),sarcoma S_(180) and leukemia(L_(1210)).Tumor inhibitory action and life-prolonging effect of AIDI on the mice,as well as its synergism with CTX were observed.MTT assay was used to examine the growth rates rates inhibition of AIDI on human hepatoma(BEL7402),human cervical carcinoma(HeLa),human melanoma(A375-S2),human gastric carcinoma(SGC-7901) and mice fibroma sarcomatosum(L_(929)).Results The growth rates of solid tumor of mice bearing S_(180) and H_(22) were obviously inhibited by AIDI at the doses of 0.972 g·kg~(-1)、1.943 g·kg~(-1) and 3.886 g·kg~(-1) i.v.for seven days.AIDI showed significant synergism effect with CTX in the growth inhibition of S_(180) in mice.AIDI could inhibit the growth of various human tumor lines in vitro.The IC_(50) of AIDI on various tumor line were obtained.Conclusions AIDI has antitumor activity in vivo and in vitro.