Fluorescent Magnetic Bioprobes by Surface Modification of Magnetite Nanoparticles |
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| Authors: | Paula C. Pinheiro Ana L. Daniel-da-Silva Daniela S. Tavares M. Pilar Calatayud Gerardo F. Goya Tito Trindade |
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| Affiliation: | 1.Department of Chemistry-CICECO (Centre for Research in Ceramics and Composite Materials), Aveiro Institute of Nanotechnology, University of Aveiro, 3810-193 Aveiro, Portugal; E-Mails: (P.C.P.); (A.L.D.S.); (D.S.T.);2.Institute of Nanoscience of Aragón (INA), University of Zaragoza, 50018 Zaragoza, Spain; E-Mails: (M.P.C.); (G.F.G.) |
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| Abstract: | Bimodal nanoprobes comprising both magnetic and optical functionalities have been prepared via a sequential two-step process. Firstly, magnetite nanoparticles (MNPs) with well-defined cubic shape and an average dimension of 80 nm were produced by hydrolysis of iron sulfate and were then surface modified with silica shells by using the sol-gel method. The Fe3O4@SiO2 particles were then functionalized with the fluorophore, fluorescein isothiocyanate (FITC), mediated by assembled shells of the cationic polyelectrolyte, polyethyleneimine (PEI). The Fe3O4 functionalized particles were then preliminary evaluated as fluorescent and magnetic probes by performing studies in which neuroblast cells have been contacted with these nanomaterials. |
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| Keywords: | magnetic nanoparticles fluorescence polyelectrolyte surface functionalization |
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