| 双分沁功能IgM、IgG2a单克隆抗体杂交瘤细胞株的建立与鉴定 |
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| 引用本文： | 林学颜,陈晓松,钮泽春.双分沁功能IgM、IgG2a单克隆抗体杂交瘤细胞株的建立与鉴定[J].细胞与分子免疫学杂志,1990(4). |
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| 作者姓名： | 林学颜 陈晓松 钮泽春 |
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| 作者单位： | 卫生部武汉生物制品研究所细胞工程研究室，卫生部武汉生物制品研究所细胞工程研究室，卫生部武汉生物制品研究所细胞工程研究室 武汉 430060，武汉 430060，武汉 430060 |
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| 摘 要： | 应用HB_sA_g采取两种不同的免疫方法免疫小鼠后。取免疫的脾细胞与小鼠骨髓瘤细胞Sp2/0融合,筛选出两株抗HB_s的单克隆抗体杂交瘤细胞株。常规免疫方法获得的细胞株为WHB_s1,分泌IgG1。脾内免疫方法获得的细胞株WHB_s2,分泌IgM和IgG_(2a)抗体。经过3个月的连续培养,能稳定分泌效价高,特异性强的单克隆抗体。
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| 关 键 词： | HB_sAg 脾内免疫 双分泌 抗HB_s |
Establishment and Characterization of Monoclonal Antibody Hybridoma Cell Line with Double Secreting Capacity (IgG_(2a), IgM) |
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| Lin Xueyan,et al.Establishment and Characterization of Monoclonal Antibody Hybridoma Cell Line with Double Secreting Capacity (IgG_(2a), IgM)[J].Journal of Cellular and Molecular Immunology,1990(4). |
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| Authors: | Lin Xueyan |
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| Institution: | Wuhan Institute of Biological Products Wuhan 430060 |
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| Abstract: | Using two different immunization methods, two hybridoma cell lines(WHBs1, WHBs2) secreting antibodies against HBsAg were established by fusing mur-ine myeloma cell SP2/0 with spleen cells from BALB/c mice immunized with HBsAg. WHBsl, which was established with conventional immunizing method, is able to secret IgGl antibody, and WHBs2, which was established with intra spleen immunizing method, has the capacity to secrete both IgG2a and IgM antibodies. The specificity and titer of the monoclonal antibodies were not influenced for 3 months in culture. |
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| Keywords: | HBsAg anti-HBs McAb double secretjimmunization intra spleen |
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