杠柳苷对BT_549细胞增殖的抑制作用与p16、p27表达关系的研究

背景与目的： 观察杠柳苷对人乳腺癌细胞株BT_549体外增殖抑制的作用，检测p16和p27的表达，探讨杠柳苷抑制乳腺癌细胞的可能作用机制。 材料与方法： 以不同浓度的杠柳苷(2.5、5.0、10.0 μg/ml)干预BT_549细胞，并设空白对照组,干预24、48、72 h后，分别用MTT比色法分析细胞增殖情况；流式细胞技术检测细胞凋亡与周期分布。半定量RT_PCR、流式细胞技术检测细胞p16、p27 mRNA和蛋白表达水平。 结果： 与对照组相比较，杠柳苷各实验组BT_549细胞增殖水平较对照组明显下降(P<0.01)，促进细胞凋亡率明显增加(P<0.01)，实验组细胞发生G0/G1期阻滞，细胞的p16、p27表达水平显著增高(P<0.01)。 结论： 杠柳苷具有抑制BT_549细胞增殖的作用，其机制可能与促进p16和p27表达水平的增高有关。

The Relationship between Suppressive Effects of Cortex Periplocae on BT-549 Cells and Expression of p16 and p27

BACKGROUND AND AIM: The suppressive effects of cortex periplocae on human breast cancer BT-549 cells and the expressions of p16 and p27 were analyzed to demonstrate the possible mechanisms. MATERIALS AND METHODS: The suppressive effects of Cortex periplocae on proliferation of BT-549 cells was analyzed with MTT method. The cell cycle and apoptosis rate of BT-549 cells treated with Cortex periplocae were examined by flow cytometry. Expressions of p16 and p27 mRNA and proteins were assessed by semiquauntitative RT-PCR and flow cytometry. RESULTS: Cortex periplocae could obviously inhibit proliferation of BT-549 cells (P<0.01). Compared to control group, after treatment with Cortex periplocae, the cell number in G0/G1 phase of BT-549 cells was increased(P<0.01), the apoptosis rate was also increased significantly(P<0.01) as well as the expression of p16 and p27(P<0.05). CONCLUSION: Cortex periplocae exerted significant inhibitory effects on BT-549 cells in vitro which was probably related to improving the expressions of p16 and p27.