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Intragranular colocalization of CRF and Met-Enk-8 in nerve terminals in the rat median eminence |
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| Authors: | Setsuji Hisano Shigeo Daikoku Noboru Yanaihara Tamotsu Shibasaki |
| Affiliation: | 1Department of Anatomy, School of Medicine, The University of Tokushima, Tokushima 770, Japan 2Laboratory of Bioorganic Chemistry, Shizuoka College of Pharmacy, Shizuoka 422, Japan 3Department of Medicine, Tokyo Women's Medical College, Tokyo 162Japan |
| Abstract: | The ultrastructural localization of rat corticoliberin (rCRF) and methionine-enkephalin octapeptide (Met-Enk-8) in the external layer of the rat median eminence were examined by double-immunogold labeling with anti-rCRF and anti-Met-Enk-8 sera labeled with small-sized (Gs) and medium-sized (Gm) gold particles, respectively. Two types of immunolabeled terminals were distinguished: one type with small granules (70 nm) labeled with Gs, and the other with large granules (100 nm) labeled with Gm. In both types, however, some granules were labeled with both Gs and Gm. |
| Keywords: | electron microscopy double-immunogold labeling corticoliberin Met-Enk-8 coexistence median eminence rat |
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