Nucleotide sequence of a Japanese isolate of Squash mosaic virus. Brief report |
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Authors: | Han S S Yoshida K Karasev A V Iwanami T |
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Institution: | (1) National Institute of Fruit Tree Science, Tsukuba, Ibaraki, Japan, JP;(2) Apple Research Center, National Institute of Fruit Tree Science, Morioka, Iwate, Japan, JP;(3) Biotechnology Foundation Laboratories at Thomas Jefferson University, Department of Microbiology and Immunology, Philadelphia, Pennsylvania, U.S.A., US |
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Abstract: | Summary. The complete nucleotide sequence of the RNA-1 of Squash mosaic virus (SqMV) was determined using a Japanese isolate (Y-SqMV). The sequence consisted of 5865 nucleotides excluding the poly (A)
at the 3′ terminus and contained a single long open reading frame with a coding capacity for a protein of Mr209971. Analysis of the deduced amino acid sequence suggested a genomic organization typical of comoviruses. The nucleotide
sequence of the RNA-2 of Y-SqMV was also determined and compared with the SqMV isolates from the United States. The larger
and smaller capsid protein (CP) coding region was compared to those of K-SqMV and Z-SqMV, which represent two subgroups of
SqMV. The larger CP gene of Y-SqMV showed 93.0% and 88.0% identities with those of K-SqMV and Z-SqMV, respectively at the
nucleotide level. The smaller CP gene of Y-SqMV was 94.1% and 88.4% identical with those of K-SqMV and Z-SqMV. The results
suggested that the Japanese SqMV isolate (Y-SqMV) is distinct from those in the United States, and might represent a third
subgroup.
Received May 7, 2001 Accepted August 1, 2001 |
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