RP-HPLC法同时测定雪莲注射液中3种成分的含量

目的建立雪莲注射液中绿原酸、芦丁和紫丁香苷的含量测定方法。方法采用Hypersil BDSC-18色谱柱,以乙腈(流动相A)和50 mmol.L-1KH2PO4缓冲溶液(含体积分数为0.2%的三乙胺,H3PO4调pH=3.6)(流动相B)进行梯度洗脱,流速为1 mL.min-1,检测波长为260 nm。结果样品中绿原酸、紫丁香苷和芦丁的回收率分别为w=99.6%、99.5%和99.0%,RSD分别为0.76%0、.86%和0.96%,绿原酸、紫丁香苷和芦丁的线性分别为0.018~0.360 g.L-1、0.008~0.150 g.L-1和0.007~0.130 g.L-1。结论该方法可用于雪莲及其注射液的质量控制。

RP-HPLC determination of three components in Xuelian injection

Objective To develop an RP-HPLC method for the determination of chologenic acid,rutin and syringin in Xuelian(traditional Chinese medicines) injection.Methods A BDS C_(18) and a gradient elution with acetonitrile-phosphate buffer as the mobile phase were employed.The flow rate was (1 mL· min~(-1)) and the detection wavelength was set at (260 nm).Results The linear range of chologenic acid was (0.018 0～)(0.360 g·L~(1)) with a regression coefficient of r=(0.999 9),the average recovery was 99.6% with RSD of 0.76%;the linear range of syringin was (0.007 5～)(0.15 g·L~(-1)) with a regression coefficient of (r=)(0.999 9),the average recovery was 99.5% with RSD of 0.86%;the linear range of rutin was (0.006 5～)(0.130 0 g·L~(-1)) with a regression coefficient of (r=)(0.999 5),the average recovery was (99.0%) with RSD of (0.96%).Conclusions This method is proved to be sensitive and accurate,and is suitable for the quality control of Xuelian injection.