葡萄糖苷和半乳糖苷的鉴定——正丁基硼酸衍生物的快原子轰击质谱

本文为葡萄糖苷和半乳糖苷的鉴定提供了一种快速、简便的新方法。即采用能够与糖中不同位置的羟基发生立体选择性反应的正丁基硼酸做为衍生试剂,对12个葡萄糖苷和半乳糖苷进行化学衍生反应,对其产物进行快原子轰击质谱分析,根据谱图的特征,可以区分葡萄糖苷和半乳糖苷。

IDENTIFICATION OF GLUCOSIDES AND GALACTOSIDES AS N-BUTANEBORONATE DERIVATIVES BY FAST ATOM BOMBARDMENT MASS SPECTROMETRY

Fast atom bombardment mass spectrometry was applied to the identification of glucosides and galaetosides as n-butaneboronate derivatives. The stereoselective reaction of six glucosides and six galaetosides——1-O-methyl α-D-glucopyranoside, 1-O-methyl α-D-galactopyranoside; 1-deoxy-l-phenyl β-D-glucoside. 1-deoxy-l-phenyl β-D-galactoside; 1-deoxy-p-methoxy-phenyl β-D-glueoside, 1-deoxy-p-methoxy-phenyl β-D-galactoside; 4-methylumbelliferone β-glucoside, 4-methyl-umbelliferone β-galactoside; 4′-hydroxy-flavone-7-β-glucoside, 4′-hydroxyflavone-7-β-galactoside; O-nitrophenyl-β-D-galactopyranoside and puerarin——have been studied by fast atom bombardment mass spectrometry. The characteristic mass spectra of the derivatized glucoside and galactoside showed differences which could be used in the differentiation of the stereoisomers in fast atom bombardment mass spectrometry.