电针对中枢Stat5敲除所致肥胖小鼠瘦素水平和下丘脑Kctd15、Sh2b1 mRNA的影响

目的 观察电针对中枢Stat5条件性敲除Stat5 Nestin-Cre (Stat5NKO)肥胖小鼠下丘脑Kctd15、Sh2b1基因表达的影响。方法 20只肥胖的Stat5NKO小鼠随机分为肥胖组和电针组，每组10只；10只Stat5fl/fl小鼠作为正常组。其中电针组选取单侧后三里、内庭穴进行针刺治疗，每日1次，双侧交替进行，每次30 min，韩氏电针仪频率2/15 Hz，疏密波，6 d为1个疗程，共治疗4个疗程。运用ELISA法检测血清中瘦素（Leptin）含量，qPCR技术检测下丘脑中肥胖相关基因Kctd15、Sh2b1 mRNA的表达。结果 与正常组小鼠比较，肥胖组小鼠体质量明显增加(P<0.01)伴随血清Leptin含量升高(P<0.05)，下丘脑Leptin水平下降(P<0.05)，同时下丘脑Kctd15、Sh2b1 mRNA表达下调(P<0.05~0.01)；与肥胖组小鼠比较，电针组小鼠的体质量明显下降(P<0.01)，血清Leptin含量降低(P<0.01)，且下丘脑Leptin水平回升(P<0.01)，Kctd15、Sh2b1 mRNA表达水平上调(P<0.01)。结论 电针可能通过调节Leptin水平并促进Kctd15、Sh2b1表达，实现对Stat5NKO小鼠的减肥效应。 

Effects of Electro-acupuncture on Serum Leptin Content and Expression of Kctd15 and Sh2b1 mRNA in Hypothalamus in Stat5-knockout Induced Obese Mice

OBJECTIVE To observe the effect of electro-acupuncture on central conditional knockout Stat5 Nestin-Cre obese mice (Stat5NKO) and detect how the obesity related genes， Kctd15 and Sh2b1， are involved. METHODS 20 obese Stat5NKO mice were randomly divided into two groups: obese model group (n=10) and electro-acupuncture （EA） group (n=10); 10 Stat5fl/fl mice were randomly selected and fed with ordinary forage as normal control group. EA group was treated with electro-acupuncture at Housanli and Neiting points for 30 min， with bilateral alternates， by Han' electro-acupuncture apparatus at 2/15 Hz. 6 d was a period of treatment， with a total of 4 courses of treatment. Serum Leptin content was measured by ELISA， and the levels of Sh2b1 and Kctd15 mRNA in hypothalamus were detected by qPCR. RESULTS Compared with Stat5fl/fl mice， Stat5NKO mice gained body weight significantly (P<0.01)， accompanied with increased serum Leptin level (P<0.05)， decreased hypothalamic Leptin level (P<0.05)， as well as Kctd15 and Sh2b1 mRNA expressions (P<0.05~0.01); Compared with Stat5NKO mice， EA group decreased body weight (P<0.01) and serum Leptin mRNA level (P<0.01). The hypothalamic Leptin level was elevated (P<0.01)， and the expressions of Kctd15 and Sh2b1 mRNA were returned to normal level (P<0.01). CONCLUSION The weight-loss effect of electro-acupuncture on Stat5NKO obese mice may be related to the regulation of the expression of Leptin， Kctd15 and Sh2b1.