| 磷脂酰肌醇3-激酶/蛋白激酶B磷酸化是胰岛素受体后信号转导通路控制PC12细胞凋亡的机制 |
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| 引用本文： | 郭丽萍,王坚,蒋雨平. 磷脂酰肌醇3-激酶/蛋白激酶B磷酸化是胰岛素受体后信号转导通路控制PC12细胞凋亡的机制[J]. 中国临床神经科学, 2007, 15(6): 596-599 |
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| 作者姓名： | 郭丽萍 王坚 蒋雨平 |
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| 作者单位： | 1. 北京中日友好医院神经内科,100029
2. 复旦大学附属华山医院神经科,200040 |
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| 摘 要： | 目的:研究胰岛素抵抗1-甲基-4苯基砒啶(MPP )诱导的PC12细胞凋亡的信号转导途径中,磷脂酰肌醇3-激酶/蛋白激酶B(PI3-K/PKB)的活力变化。方法:应用Wortmannin(PI3-K抑制剂),比较用药前后细胞生存率的变化;应用Western印迹分析检测此间PKB及其磷酸化水平的变化。结果:Wortmannin预处理组细胞生存率较之胰岛素干预组明显下降;PKB的特异性磷酸化程度(Ser473磷酸化程度/激酶蛋白量)与细胞生存率的变化有关。结论:胰岛素主要通过调节PI3-K活性后再促进PKB的磷酸化,从而促进PKB的激活,并导致生物学效应的变化,但是尚不能排除其他机制的参与。
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| 关 键 词： | 胰岛素 胰岛素受体 凋亡 PC12细胞 磷脂酰肌醇3-激酶/蛋白激酶B 1-甲基-4苯基砒啶 |
| 文章编号： | 1008-0678(2007)06-0596-04 |
| 修稿时间： | 2007-01-09 |
The Phosphorylation of Phosphatidylinositol 3''''-OH Kinase/Protein Kinase B is an Effective Molecule in Insulin-Insulin Receptor Signal Transduction Against Apoptosis of PC12 Cells |
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| GUO Li-Ping,WANG Jian,JIANG Yu-Ping. The Phosphorylation of Phosphatidylinositol 3''''-OH Kinase/Protein Kinase B is an Effective Molecule in Insulin-Insulin Receptor Signal Transduction Against Apoptosis of PC12 Cells[J]. Chinese Journal of Clinical Neurosciences, 2007, 15(6): 596-599 |
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| Authors: | GUO Li-Ping WANG Jian JIANG Yu-Ping |
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| Abstract: | Aim:To study the effect of PI3-K/PKB in the pathway of insulin-insulin receptor signal transduction against apoptosis of PC12 cells. Methods:With Wortmannin the specific inhibitor of PI3-K,compared the change of the PC12 cells viability;with Western immunoblotting method,analysed the change of the phosphorylation of PKB. Results:Cell treated with Wortmannin exhibited lower cells viability than insulin-treated. The phosphorylation of PKB at Serine(Ser) 473 residues altered in different experiment conditions accordingly. Conclusion:The phosphorylation of PKB was an important molecule against MPP -induced apoptosis in PC12 cells |
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