真核表达单纯疱疹病毒糖蛋白模拟表位mgC及其基因免疫效果的观察

目的 鉴定单纯疱疹病毒（HSV）糖蛋白C(gC)一个短肽模拟表位mgC基因作为HSV DNA表位疫苗的可能性。方法 将mgC基因克隆入真核载体pcCNA3.1中，并在CHO细胞中表达，免疫荧光法鉴定表达效果。DNA免疫不同种属小鼠，ELISA法观察HSV gC特异结合抗体的诱生。结果 含mgC的真核载体在哺乳动物细胞中可以成功表达外源蛋白。重组载体DNA在BALB／c小鼠体内可诱导抗天然HSV gC蛋白的抗体，而在C57BL鼠体内未见明显的抗体反应。结论 成功构建了可表达模拟表位mgC的真核表达载体。证明了应用gC模拟短肽基因作为DNA免疫的基因源是可行的，但其对不同种属动物的反应性不同，提示DNA免疫还应针对不同免疫对象调整免疫策略。这一研究为HSV多表位组合多肽疫苗的研制提供了一个新的备选组份。

Expression and Immunogenicity of the eukaryotic vector of mimotope mgC derived from glycoprotein C of Herpes simplex virus

Aim To identify the possibility that the eukaryotic recombi-nant expression vector containing a mimic epitope gene(mgC)can be used as a DNA epitope vaccine of herpes simplex virus(HSV).Methods Cloned the oligonucleotide encodin g the HSV mimotope into the multiple cloning site of pcDNA3.1.Transfected the recombinant vector mgC /pcDNA into CHO cells,and then detected expression of the minigene by IFA.Immunized 2types of mice with th e purified plasmid and the spe-cific antibody level was measured by ELISA.Results the mgC minigene can be expressed exactly in CHO cells.And the combinant DNA could elicits Balb /c mice antibody directly against HSV gC in the sera,but the relativity antibody can not be measu red in C57BL mice.Conclusion The recombinant mgC gene clone has been c onstructed successfully,and it also can be used as DNA immunogen.Sin ce the antibody levels elicitedin different stran of mice are different,it imply that immunization strategies should be adjusted,resp ectively.This study may provide a novel epitope for preparing multi-e pitope peptide vaccine of HSV.