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| Bestatin下调卵巢癌患者外周血Treg细胞的免疫抑制作用 | |
| 引用本文： | 陆恬,葛彦,居颂文,蒋林华,张弛,孙雪薇,居颂光.Bestatin下调卵巢癌患者外周血Treg细胞的免疫抑制作用[J].中国血液流变学杂志,2009,19(4):506-508. |
| 作者姓名： | 陆恬 葛彦 居颂文 蒋林华 张弛 孙雪薇 居颂光 |
| 作者单位： | 1. 苏州市立医院东区妇产科,江苏苏州,215001 2. 苏州大学医学部基础与生物科学学院免疫学系,江苏苏州,215123 3. 江苏省人民医院肿瘤中心,江苏南京,210029 |
| 基金项目： | 国家自然科学基金青年科学基金资助项目 |
| 摘 要： | 目的探讨氨肽酶N抑制剂Bestatin对肿瘤患者外周血CD4^＋CD25^＋Treg的生物学效应及机理。方法将Bestatin加入PHA刺激新鲜分离的卵巢癌患者外周血Treg细胞的体外培养体系，采用流式细胞术分析Foxp3和CTLA-4的表达。进而分析Bestatin对Treg细胞抑制CD4^＋CD25T细胞活化增殖的影响。结果Bestatin可抑制Treg胞表达Foxp3和CTLA-4，并促进Treg细胞和CD4^＋CD25^＋T细胞的体外共培养体系中T细胞的增殖。结论抑制氨肽酶N可下调Treg细胞的免疫抑制作用，对肿瘤治疗具有潜在应用价值。 |
| 关 键 词： | 氨肽酶N CD13 Bestatin Treg |
Bestatin Inhibits the Suppressive Function of Treg Cells from Peripheral Blood of Ovarian Cancer Patients |
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| LU Tian,GE Yan,JU Song-wen,JIANG Lin-hua,ZHANG Chi,SUN Xue-wei,JU Song-guang.Bestatin Inhibits the Suppressive Function of Treg Cells from Peripheral Blood of Ovarian Cancer Patients[J].Chinese Journal of Hemorheology,2009,19(4):506-508. | |
| Authors: | LU Tian GE Yan JU Song-wen JIANG Lin-hua ZHANG Chi SUN Xue-wei JU Song-guang |
| Institution: | LU Tian,GE Yan,JU Song-wen, JIANG Lin-hua,ZHANG Chi,SUN Xue-wei, JU Song-guang (1 .Obstetrics and Gynecology Department, East District of Suzhou Municipal Hospital,Suzhou,Jiangsu,215001; 2. Immunology Department,School of Medicine,Soochow University,Suzhou,Jiangsu,215123;3. Cancer Center, the First Affiliated Hospital of Nanjing Medical University,Nanjing, Jiangsu,210029) |
| Abstract: | Objective To explore the biological effect of Bestatin,CD13 specific inhibitor, and its molecular mechanism on CD4^＋CD25^＋Treg cells.Methods Bestatin was added to stimulate the T cells that isolated from peripheral blood of ovarian cancer patients and activated by PHA.T cell proliferation was determined by cell counting at 3d. The CTLA-4 expression of Treg cells was determined by FACS,and Foxp3 analysis was performed after fixation and intracellular staining.Results Bestatin could decrease the expression of CTLA-4 and Foxp3 of CD4^＋CD25^＋Treg cells and suppress the ability of Treg cells to inhibit proliferation of CD4^＋CD25^＋T cells that activated by PHA. Conclusion Bestatin could inhibit the suppressive function of Treg cells,and CD 13 might be a potential molecular target for the immunological interference. |
| Keywords: | CD13 Bestatin Treg |
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