95%乙醇浸泡异体肌腱移植的实验研究

目的：研究用９５％乙醇浸泡处理后的异体肌腱移植对肌腱愈合的影响。方法：选用ＳＤ大鼠８０只，随机分为３组。乙醇浸泡异体肌腱组：切除大鼠跟腱全长，用９５％乙醇浸泡处理的异体肌腱桥接于腱缺损处。新鲜异体肌腱组：用新鲜异体肌腱移植于腱缺损处。自体肌腱组：用自体肌腱移植于腱缺损处，作为对照组。各组分别于术后２、３、４、６周观察肌腱形态学和组织学的变化；术后半年进行肌腱拉力试验。结果：乙醇浸泡处理的异体肌腱有明显降低组织抗原性的作用，移植后对肌腱愈合无明显影响，拉力和对照组相似。结论：乙醇浸泡处理后的异体肌腱可应用于临床

An experimental study on the 95% ethanol immersed allogenous tendon graft

Objective:To investigate the effect of 95% ethanol immersion processing on tendon healing in allogenous tendon grafting.Methods:80 SD rats were randomly divided into 3 groups.In 95% ethanol immersed allogenous tendon group,the achilles tendons were harvested and immersed in 95% ethanol before being grafted to the defect site.In the fresh allogenous tendon group and autogenous tendon group,the fresh allogenous and autogenous tendons were interposed at the defect sites respectively.The morphological and histological changes were observed at 2,3,4,and 6 weeks postoperatively.Loading test of the grafted tendons was carried out at the sixth postoperative month. Results:Ethanol processing of the allogenous tendons can remarkably reduce their antigenicity without affecting tendon healing and tensile strength.Conclusion:The ethanol immersion processed allogenous tendons can be used in clinical practice.