单侧梨状脑中间区内微量注射生理盐水对大鼠杏仁核电点燃癫痫大发作的影响

目的:探讨在大鼠梨状脑中间区微量注射生理盐水对杏仁核电点燃癫痫大发作的影响。方法:大鼠每日进行杏仁核电点燃直至达到大发作状态,随后在单侧梨状脑中间区内注射不同体积的生理盐水,并在注射后10min、30min、1d、4d、7d、10d观察大发作的发生率和阈值变化,以及持续时间的变化。结果:在同侧或对侧梨状脑中间区内注射0.1μl和0.25μl及1μl的生理盐水,均能剂量依赖性地降低癫痫大发作发生率,同时显著缩短大发作持续时间(P<0.05),而且这种作用可持续10d。同侧组在注射各个剂量生理盐水10min后即可显著升高GST(P<0.05),持续10d,而对侧组在注射剂量为0.1μl的生理盐水30min后才显著升高GST(P<0.05)。结论:单侧梨状脑中间区内注射生理盐水能够抑制大鼠杏仁核电点燃癫痫大发作,提示可能成为一种对颞叶癫痫有效的治疗手段。

Injection of saline into unilateral central piriform cortex inhibits amygdaloid-kindled seizures in rats

OBJECTIVE: To investigate the effect of microinjection of saline into unilateral central piriform cortex (cPC) on the generalized seizures in amygdaloid-kindled rats. METHODS: Different volumes of saline were injected into the right or left cPC of amygdaloid-kindled rats, and its effect on generalized seizures was observed. RESULT: Saline injection at different volumes 0.1 microl, 0.25 microl and 1 microl) significantly decreased the incidence and duration of generalized seizures (P<0.05), the anticonvulsant effect lasted for up to 10 d. In addition, 10 min after ipsilateral injection of saline the generalized seizure thresholds were significantly increased; while this effect was observed 30 min later when contralateral injection was used. CONCLUSION: Unilateral saline injection into cPC has a significant anticonvulsant effect, which might be used for treatment of human temporal lobe epilepsy in the future.