可逆性咽鼓管阻塞建立豚鼠急性分泌性中耳炎模型

目的 探索单纯咽鼓管阻塞建立急性分泌性中耳炎豚鼠动物模型的方法,为分泌性中耳炎急性期的相关实验研究提供一种理想的动物模型.方法 健康雄性豚鼠22只,左侧为实验耳,右侧为对照耳,经软腭切开膨胀海绵阻塞左侧咽鼓管咽口建立可逆性分泌性中耳炎动物模型,耳内镜下观察记录鼓膜形态和鼓窜积液情况,分别于术后第7天、第14天及第21天各获取6只造模成功的豚鼠听泡,探查术后膨胀海绵留置情况,HE染色观察病程中中耳黏膜的病理改变.结果 造模组22耳中20耳分别于术后第3～7天出现鼓室浆液性渗出,造模成功率为90.9%,病理改变表现为鼓室黏膜增厚,黏膜下血管扩张,淋巴细胞浸润,近咽鼓管鼓室口部纤毛低矮,数量减少甚至脱落.术后14 d膨胀海绵已基本排出,14～18 d中耳积液消失,同时组织形态逐渐恢复正常.病程中对侧对照耳鼓室无积液,亦无明显病理改变.结论 经软腭切开膨胀海绵咽鼓管阻塞法可成功建立豚鼠急性分泌性中耳炎模型,该分泌性中耳炎可于膨胀海绵自行排出后逐渐自愈.

Abstract：

Objective To develop a animal model for acute otitis media with effusion (OME). Methods In 22 guinea pigs, the left nasal orifice of Eustachian tube was approached via a transpalatal incision and obstructed with polyvinyl acetal material. Right ears were set as the control. Then all the ears were evaluated by otomicroscopy every day. Seven, 14 and 21 days after the intervention, six guinea pigs were killed for histologic study. Results Of the 22 guinea pigs included in this study, 20 ears (90. 9% ) were found to have effusion 3-7 days after the operation, two cases were excluded for purulent otorrhea 10 days postoperatively. The epithelium initially developed hyperplasia, and the submucosa showed vascular and lymphatic dilatations with inflammatory cells infiltration. None of the contralateral control ears had evidence of disease by otomicroscopic examination and histologic study. Conclusion This experimental methods provoked reproducible pathologic characteristics similar to those for otitis media with effusion.

A guinea pigs model of otitis media with effusion caused by reversible Eustachian tube obstruction

Objective To develop a animal model for acute otitis media with effusion (OME). Methods In 22 guinea pigs, the left nasal orifice of Eustachian tube was approached via a transpalatal incision and obstructed with polyvinyl acetal material. Right ears were set as the control. Then all the ears were evaluated by otomicroscopy every day. Seven, 14 and 21 days after the intervention, six guinea pigs were killed for histologic study. Results Of the 22 guinea pigs included in this study, 20 ears (90. 9% ) were found to have effusion 3-7 days after the operation, two cases were excluded for purulent otorrhea 10 days postoperatively. The epithelium initially developed hyperplasia, and the submucosa showed vascular and lymphatic dilatations with inflammatory cells infiltration. None of the contralateral control ears had evidence of disease by otomicroscopic examination and histologic study. Conclusion This experimental methods provoked reproducible pathologic characteristics similar to those for otitis media with effusion.