PBK/TOPK在TPA诱导的人HL-60细胞分化中的表达及意义

目的:研究PBK/TOPK在TPA(佛波酯)诱导白血病细胞HL-60的分化时的表达及意义.方法:细胞化学染色法观察药物作用后细胞形态的变化;流式细胞仪检测药物对HL-60细胞周期及细胞表面分化抗原CD11b,CD14,CD13和CD33表达的影响;用western Blot法检测PBK/TOPK在HL-60细胞分化前后表达的变化.结果:经5.1 nmol/L TPA处理72 h后,NBT阳性细胞数和CD11b,CD13,CD14表达是增加的,HL-60细胞体积缩小,核浆比例减低,核仁减少甚至消失,核形态扭曲折迭或分叶;PBK/TOPK在HL-60细胞向成熟分化后表达下调.结论:TPA能抑制白血病细胞HL-60的增殖,并诱导其向成熟单核、粒细胞分化,在此过程中,PBK/TOPK表达是下调的,但Pho-PBK/TOPK的表达是增加的.

Expression and significance of PBK/TOPK in differentiation of human HL-60 leukemic cells induced by TPA

AIM: To investigate the expression and significance of PBK/TOPK in the differentiation of human HL-60 leukemic cells induced by TPA. METHODS: After induction of TPA, Wright-Giemsa staining was performed to observe the morphological changes of HL-60 cells and flow cytometry was used to observe the cell cycles and the expressions of CD11b, CD14, CD13, CD33, respectively. Expression of PBK/TOPK was detected by Western Blot. RESULTS: After the treatment with 5.1 nmol/L TPA for 3 d, the number of NBT positive cells and the expressions of CD11b, CD13 and CD14 increased, and the expression of PBK/TOPK decreased. CONCLUSION: TPA can inhibit the proliferation of HL60 cells and induce the differentiation of HL60 cells into the granulocytic or monocytic lineage. During the process, PBK/TOPK expression is down-regulated and the expression of Pho-PBK/TOPK is up-regulated.