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BASIC FIBROBLAST GROWTH FACTOR GENE TRANSFECTION TO ENHANCE THE REPAIR OF AVASCULAR NECROSIS OF THE FEMORAL HEAD |
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| Authors: | Cao Yang Shu-hua Yang Jing-yuan Du Jin Li Wei-hua Xu Yu-fang Xiong |
| Institution: | Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022. yangcaom@sina.com |
| Abstract: | Objective To explore a new method for the therapy ofavascular necrosis of the femoral head.Method The recombinant plasmid pCD-rbFGF was mixed with collagen and was implanted in the necrotic femoral head. Expression of basic fibroblast growth factor (bFGF) was examined by RT-PCR and immunohistochemical method. Repair of the femoral head was observed by histological and histomorphometric analysis.Result Expression of bFGF was detected in the femoral head transfected with bFGF gene, indicating significant increase of angiogenesis 2 weeks after gene transfection and increased new bone formation 8 weeks after gene transfection on histomorphometric analysis (P ＜ 0.01).Conclusion Transfection ofbFGF gene enhances bone tissue angiogenesis. Repair in osteonecrosis would be accelerated accordingly. |
| Keywords: | basic fibroblast growth factor gene transfection avascular necrosis femoral head FEMORAL HEAD AVASCULAR NECROSIS REPAIR GENE TRANSFECTION FIBROBLAST GROWTH FACTOR osteonecrosis would Transfection tissue significant increased angiogenesis bone formation gene transfection Result Repair analysis immunohistochemical method bFGF |
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