MOBP基因在注意缺陷多动障碍大鼠前额叶皮层表达的研究

目的:探讨注意缺陷多动障碍(ADHD)动物模型SHR大鼠前额叶皮层MOBP基因表达情况,分析其在ADHD病理生理学中的意义.方法:应用RT-PCR、免疫组化、电镜技术检测该基因mRNA、蛋白表达情况以及轴突髓鞘形态变化.结果:RT-PCR结果显示SHR大鼠脑组织中MOBP mRNA表达水平(35.78±6.04)%明显低于正常对照WKY大鼠(69.41±7.43)%(P＜0.01);免疫组化结果提示SHR大鼠前额叶皮层髓鞘着色显著变浅、稀疏,着色面积占总面积比例(7.43±3.51)%较对照组WKY(14.39±5.82)%明显减少(P＜0.05);电镜观察结果显示,部分SHR大鼠髓鞘放射性结构呈直线性分布.结论:MOBP基因在SHR中的表达下调可能通过参与损害中枢神经系统髓鞘结构和功能,在ADHD病理机制中发挥作用.

Expression of MOBP in the PFC of Animal Model of ADHD in SHR

Objective:To explore the changes of the myelin-associated oligodendrocytic basic protein(MOBP) gene mRNA and protein expression in the prefrontal cortex(PFC) of SHR/WKY and to analyze the relationship between the MOBP and the etiology and pathophysiology of Attention Deficit Hyperactivity Disorder(ADHD). Methods:The expressive levels of MOBP in the PFC of SHR and WKY were verified by using RT-PCR technique. The distribution of the MOBP in rat brains was marked by used MOBP-HRP immunohistochemistry(IHC) and the electron microscope were applied as the ultrastructural study on brain tissues. Results:The levels of MOBP gene mRNA expression(35.78 ± 6.04)% in the PFC of SHR were significantly lower than those(69.41 ± 7.43)% in the PFC of WKY rats. At the same time,there was ultrastructural difference in arrangement of the radial component in myelin between two groups. Conclusion:The less of MOBP expression in the SHR may be one of the important pathophysiological bases for ADHD.