趋化因子CCL22对肺癌细胞迁移和侵袭的影响

目的观察趋化因子CCL22对肺癌SBC-5细胞迁移和侵袭能力的影响。方法取肺癌细胞系SBC-5细胞,RPMI-1640培养基培养,5%CO2培养箱孵育,清洗消化后传代培养。予100ng/m L的CCL22诱导肺癌SBC-5细胞,设Control组、CCL22组(100ng/m L)、MIX组(CCL22 100ng/m L+蛋白激酶抑制剂U0126 10μmol),观察细胞迁移及侵袭能力的变化;加入蛋白激酶抑制剂(U0126)后细胞迁移及侵袭能力的变化。结果 CCL22诱导肺癌SBC-5细胞后,CCL22组细胞迁移距离(351.9±16.4)μm,明显大于Control组的(112.6±27.2)μm和MIX组的(145.1±29.6)μm(P0.05),MIX组和Control组比较差异无统计学意义(P0.05)。CCL22组平均侵袭细胞数(505.5±66.3)个,显著高于Control组的(199.2±32.8)个和MIX组的(95.7±19.1)个(P0.05),MIX组明显低于Control组(P0.05)。结论趋化因子CCL22可在体外诱导肺癌SBC-5细胞迁移和侵袭,而蛋白激酶抑制剂(U0126)可抑制CCL22诱导肺癌细胞的迁移及侵袭。

Effects of Chemokine CCL22 in lung cancer cell migration and invasion

Objective: To investigate the effect of chemokine CCL22 in lung cancer cell line SBC-5 migration and invasion. Methods: Cell-surface expression of CC chemokine receptor4(CCR4) was detected by fluorescence-activated sorting . Capability of lung cancer cell migration and invasion was viewed, after lung cancer cells line SBC-5 were induced with 100ng/ml CCL22 and were added by protien kinase inhibitor(U0126).Rusults: CCR4 was expressed in lung cancer cell-surface, and capability of lung cancer cell migration and invasion increased significantly. Protein kinase inhibitor(U0126)may inhibit lung cancer cell migration and invasion induced CCL22.Conclusion: Chemokine CCL22 may induce migration and invasion of lung cancer cell line SBC-5 in vitro.