Metaproteomics characterization of the alphaproteobacteria microbiome in different developmental and feeding stages of the poultry red mite Dermanyssus gallinae (De Geer, 1778) |
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Authors: | José Francisco Lima-Barbero Sandra Díaz-Sanchez Olivier Sparagano Robert D Finn José de la Fuente |
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Institution: | 1. SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo 12, 13071 Ciudad Real, Spain;2. Sabiotec, S.A. Ed. Polivalente UCLM Ciudad Real, Spain https://orcid.org/0000-0002-2694-0215;3. SaBio. Instituto de Investigación en Recursos Cinegéticos IREC-CSIC-UCLM-JCCM, Ronda de Toledo 12, 13071 Ciudad Real, Spain https://orcid.org/0000-0002-7205-3174;4. Department of Infectious Diseases and Public Health, City University of Hong Kong, Kowloon, Hong Kong, SAR https://orcid.org/0000-0003-3141-310X;5. Department of Applied Sciences, Faculty of Health &6. Life Sciences, Northumbria University, Newcastle Upon Tyne, UK https://orcid.org/0000-0003-0002-9711;7. Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, USA https://orcid.org/0000-0001-7383-9649 |
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Abstract: | ABSTRACTThe poultry red mite (PRM), Dermanyssus gallinae (De Geer, 1778), is a worldwide distributed ectoparasite and considered a major pest affecting the laying hen industry in Europe. Based on available information in other ectoparasites, the mite microbiome might participate in several biological processes and the acquisition, maintenance and transmission of pathogens. However, little is known about the role of PRM as a mechanical carrier or a biological vector in the transmission of pathogenic bacteria. Herein, we used a metaproteomics approach to characterize the alphaproteobacteria in the microbiota of PRM, and variations in its profile with ectoparasite development (nymphs vs. adults) and feeding (unfed vs. fed). The results showed that the bacterial community associated with D. gallinae was mainly composed of environmental and commensal bacteria. Putative symbiotic bacteria of the genera Wolbachia, C. Tokpelaia and Sphingomonas were identified, together with potential pathogenic bacteria of the genera Inquilinus, Neorickettsia and Roseomonas. Significant differences in the composition of alphaproteobacterial microbiota were associated with mite development and feeding, suggesting that bacteria have functional implications in metabolic pathways associated with blood feeding. These results support the use of metaproteomics for the characterization of alphaproteobacteria associated with the D. gallinae microbiota that could provide relevant information for the understanding of mite-host interactions and the development of potential control interventions.Research highlights Metaproteomics is a valid approach for microbiome characterization in ectoparasites. Alphaproteobacteria putative bacterial symbionts were identified in D. gallinae. Mite development and feeding were related to variations in bacterial community. Potentially pathogenic bacteria were identified in mite microbiota.
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Keywords: | Dermanyssus microbiome proteomics mite bacteria poultry pathogen |
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