不同喂养方式对婴儿肠道菌群的影响

【目的】　通过观察健康婴儿母乳及配方乳两种不同喂养方式下肠道菌群的定植过程 ,分析婴幼儿饮食与肠道菌群形成的关系并探讨其意义。　【方法】　采用荧光定量PCR技术测定细菌 16SrRNA ,经与标准曲线对照计算细菌数量 ,对 71名母乳喂养婴儿和 60名人工喂养婴儿粪便中乳酸杆菌、双歧杆菌和大肠杆菌进行了定量检测。　【结果】　婴儿肠道菌群处于动态定植过程。母乳喂养婴儿肠道乳酸杆菌、双歧杆菌数量较人工喂养婴儿高 (P<0 .0 5 ) ,而大肠杆菌数量较人工喂养婴儿低 (P <0 .0 5 )。　【结论】　荧光定量PCR法定量测定肠道菌群的数量是一种简便、快捷、特异性好的方法。婴儿期肠道菌群仍处于动态演替过程。不同的喂养方式对肠道菌群有影响 ,应大力提倡母乳喂养。

Influence of different feed fashion on intestinal microflora in infants

To investigate the difference and significance of the intestinal flora between breast feed and formula feed infants. 71 breast fed and 60 formula fed infants were studied. Real Time PCR procedures based on 16S rRNA gene sequences specific for bifidobacterium, lactobacillus and escherichia coli that predominate in the human intestinal tract were developed and used for quantitative detection of these species in infants feces. The breast fed infants were more often determined with bifidobacteria ( P <0.05),as compared with the formula fed infants. Similarly, in breast fed infants the proportions of lactobacilli were higher than in the formula fed infants. In contrast, the formula fed infants harbored higher counts of Escherichia coli ( P <0.05). Conclusions] This study confirms the differences in development of intestinal flora between breast fed and formula fed infants. The results support a preference for breast feeding over formula feeding.