动物蛛网膜下腔出血后脑血管痉挛与内皮素、一氧化氮的关系

目的:研究兔症状性蛛网膜下腔出血(ＳＡＨ)后脑血管痉挛(ＣＶＳ)与内皮素(ＥＴ)和一氧化氮(ＮＯ)的关系。方法:采用兔ＳＡＨ模型,观察ＳＡＨ前后动物进食量、神经功能和局部脑血流量(ｒＣＢＦ)改变,测定血液和脑脊液中ＥＴ和ＮＯｘ－含量。结果:ＳＡＨ后动物进食量和ｒＣＢＦ下降、神经功能障碍,血液和脑脊液中ＥＴ含量增加,ＮＯｘ－含量下降(Ｐ＜０．０１)。结论:兔双侧颈动脉结扎后枕大池二次注血可制成可靠的症状性ＳＡＨ后ＣＶＳ模型。ＳＡＨ后ＥＴ和ＮＯ含量的改变与ＣＶＳ的发生密切相关,并进而导致临床症状的恶化。

The Relationship Between Endothelin, Nitric Oxide and Experimental Cerebral Vasospasm Following Subarachnoid Hemorrhage in Rabbit

Aim:To investigate the relationship between endothelin (ET), nitric oxide (NO) and experimental cerebral vasospasm following subarachnoid hemorrhage (SAH) in rabbit. Methods: The model of SAH was set up, the food-intake and neurofunctions of experimental animals were under observation and the regional cerebral blood flow (rCBF) was measured. The contents of ET and NOx- in blood and CSF were determined. Results:The food-intake decreased in animals with SAH, and the neuroiunction disturbance appeared. rCBF decreases were also observed. The content of ET in blood and CSF increased but NOx- decreased ( P < 0.01). Conclusion: Animal model with reliable symptomatic post-SAH CVS could be experimentally induced by injecting blood two times via cisternal after occlusion of bilateral carotid. The appearance of CVS was correlative to the content of ET and NO after SAH, and then the clinical symptoms deteriorate further.