血管内皮生长因子_(165)和白细胞介素-8促兔动脉粥样硬化斑块形成作用的探讨

目的 :观察血管内皮生长因子1 6 5(VEGF1 6 5)和白介素 8(IL 8)对兔动脉粥样硬化斑块形成的影响。方法 :15只日本大耳白兔随机分为 3组 :A组为对照组 ,普通饲料喂养 ,B、C组为处理组 ,高胆固醇饲料喂养。至第 3周 ( 2 1天 ) ,A组和B组肌注白蛋白 ( 2ug/kg) ,C组肌注VEGF1 6 5( 2ug/kg) ,之后继续饲养 3周 (共 4 2天 )处死动物 ,测定各组血清IL 8和血脂 ,用计量组织学的方法测定动脉斑块内CD34的阳性细胞数。结果 :① 2 1天及 4 2天时B组和C组分别与A组相比 ,血清IL 8浓度均有显著性升高 (P <0 .0 5)。C组 4 2天与 2 1天相比 ,血清IL 8水平明显增高 (P <0 .0 1)。② 4 2天时 ,各组之间CD34阳性细胞数比较有显著差异 (P <0 .0 5)。③ 4 2天时B组和C组IL 8的水平与CD34的阳性细胞数之间呈正相关 (B组r=0 .6 4,C组r=0 .88)。④各处理组内血脂水平比较 ,2 1天与 0天相比有明显升高 (P <0 .0 1) ,4 2天与 2 1天相比无显著差异。结论 :VEGF1 6 5促进斑块内血管生成的作用可能与其促进炎性细胞分泌有关。

Effect of VEGF165 and IL-8 to Promoted on Atherosclerotic Plaque in Rabbites

Objective:To observe the effect of VEGF 165 and IL-8 on p rogression of atherosclerotic plaque in rabbit.Method:Fifteen rabbits were randomly into three groups,A group( n=5) fed with common diet and other groups(group B,n=5;group C,n=5 ) fed with high cholesterol diet.After 21 days,group A and group B were injecte d Albumin(2 μg/kg),group C was injected VEGF 165(2 μg/kg);and then to co ntinue 42 days all rabbites of three groups were killed.Their concentration of I L-8,Cho-c,LDL-C were examined and the CD 34 in as playue were counted to o.Results:①The serum IL-8 of group B and group C markly increa se comparing with group A at 21 day and 42 day;In group C levels of serum IL-8 of the 42 day is higher than the 21 day.②There are significant differences in n eovascularization density(number of CD 34 positive cells)(P<0.05)in gro u p A,B and C at 42 day.③There are positive correlation between serum IL-8 and n eovascularization density (group B,r=0.64,group C,r=0.88)in G roup B and C at 42 day.④The level of serum Cho-C and LDL-C in group B,C are all significa ntly at 21 day and 42 day. Conclusion:VEGF 165 increases angiogenesis in atherosclero tic plaque may be relative with increasing secretion of IL-8.