雷公藤甲素对急性T淋巴细胞白血病Jurkat细胞增殖和凋亡的影响

为了研究雷公藤甲素对急性T淋巴细胞白血病Jurkat细胞的增殖和凋亡效应，用不同浓度的雷公藤甲素作用于Jurkat细胞，用CCK法检测细胞存活率，选取使细胞增殖抑制率为50％的雷公藤甲素作用于细胞，然后在应用Hoechst33258染色、DNA电泳、Pl以及PI／Annexin V染色后用流式细胞仪检测细胞的凋亡。结果表明：雷公藤甲素抑制Jurkat细胞的生长增殖，半数细胞抑制剂量为4μg/L。4μg/L雷公藤甲素作用于Jurkat细胞12小时后，出现明显的细胞凋亡特征（Hoechest33258染色显示细胞核呈亮蓝色；DNA断裂产生DNA ladder，细胞凋亡的亚二倍体峰出现，细胞磷脂酰丝氨酸发生转位），细胞凋亡比率明显增加，24小时后细胞凋亡进一步增加。结论：雷公藤甲素对急性T淋巴细胞白血病Jurkat细胞有明显的抗增殖和促凋亡作用，这为临床应用雷公藤甲素治疗白血病提供了实验依据。

Effects of Triptolide on Proliferation and Apoptosis of Jurkat Cell Line in Acute T Lymphocytic Leukemia

The aim of this study was to investigate the anti-proliferation and pro-apoptosis of triptolide on Jurkat cell line in acute T lymphocytic leukemia. The Jurkat cells were treated with various concentrations of triptolide (0, 1, 2, 4, 8, 16 microg/L) for 12 hours. The inhibitory ratio was measured by Cell Counting Kit-8 assay. The effects of triptolide on apoptosis of Jurkat cells were determined by DNA fragmentation (DNA ladder), Hoechst 33258, PI and Annexin V-FITC/PI double staining. The results demonstrated that triptolide inhibited the proliferation of Jurket cells. The 50% inhibitory concentration (IC(50)) was 4.0 microg/L. Chromatin condensation in the cells treated with triptolide could be seen by light microscopy. DNA electrophoresis showed evidence of nuclear fragmentation (DNA ladder). The hypoploid (sub-G(1)) population was increased after treatment with triptolide. The translocation of phosphatidylserine at the outer surface of the cell plasma membrane could be induced by triptolide. After treatment with triptolide for 12 hours, the rates of apoptotic cells were significantly increased. Moreover, these pro-apoptosis effects were in time-dependent manner. It is concluded that triptolide can inhibit the proliferation and induce the apoptosis of Jurkat cells. This study provides experimental basis for clinical use of triptolide in leukemia therapy.