多巴胺D2受体TaqI A多态性与海洛因渴求程度的关联分析

目的探讨多巴胺D2受体TaqI A多态性与线索诱发海洛因渴求程度的关系.方法 380名海洛因依赖者接受环境诱发渴求实验,用PCR-RFLP技术检测DRD2受体TaqI A多态性,比较不同基因型与线索暴露前后渴求程度的关系. 结果强制戒毒被试者基因型与暴露前后渴求程度未见显示差异(P＞0.05).自愿戒断被试者基因型与诱发后渴求分值、暴露前后渴求分差值的差异有显著性(F=4.523,P=0.012;F=3.936,P=0.021),A1/A1基因型被试者暴露后渴求程度高于A2/A2(P=0.027,P=0.019)和A1/A2(P=0.032,P=0.035)被试者.三者基因型在成瘾、加量和海洛因使用时间差异上有显著性(P=0.014,P=0.001,P=0.004).A1/A1基因型个体成瘾时间和加量时间小于A2/A2(P=0.007,P=0.001)和A1/A2(P=0.023,P=0.001)基因型个体,但吸毒时间大于A1/A2(P=0.003)和A2/A2(P=0.002)基因型个体. 结论 DRD2受体基因TaqI A多态性可能与环境诱发海洛因渴求程度易感性和成瘾、加量及毒品使用时间有关,A2+(A2/A2,A1/A2)基因型可能在海洛因依赖中起保护作用,而A2-(A1/A1)基因型则可能具有促进作用.

Relationship between heroin craving level and DRD2 recepto gene-TaqI A polymorphism

Objective: To investigate the relationship between DRD 2 receptor TaqI A polymorphism and cue-induced craving level of heroin. Methods: Genotype of DRD 2 receptor TaqI A using PCR-RFLP were detected in 380 subiecrs with dependence. The relationship between craving level before and after and the genotype were compared. Results: No significant correlation has been found between craving level before and after cue-exposure and genotype in cue exposure forced detoxification subjects (P>0.05). However, significant correlation has been detected between genotype and cue-induced craving level and difference of crowing level befare and after cue exposure in volunteer detoxification subjects (F=4.523, P=0.012; F= 3.936, P=0.021). Craving level after cue-exposure in A 1/A 1 genotype is higher than that in A 1/A 2(P= 0.032, P=0.035) and A 2/A 2(P=0.027, P=0.019) genotype. Significant difference exists inthree different in terms of addictive time, increasing-dosage time and heroin-using time (P=0.014, P=0.001, P= 0.004). Addictive time and time of increasing-dosage in A 1/A 1 subjects are shorter than that in A 2/A 2(P= 0.007, P=0.001) and A 1/A 2(P= 0.023, P=0.001) subjects. But heroin-using time in A 1/A 1 individuals is longer than that in A 2/A 2 and A 1/A 2 individuals (P=0.002, P=0.003 respectively). Conclusion: DRD 2 receptor gene TaqI A polymorphism may have association with susceptibility of cue-induced craving level and some addictive related indexes such as addictive time, increasing-dosage time and heroin-using time. A 2 +(A 1/A 2, A 2/A 2) genotype may play a protective role in heroin dependence, whereas A 2 -(A 1/A 1) genotype take a precipitating effect.