| HPLC法测定病毒清口服液中连翘苷的含量 |
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| 引用本文： | 何春玲,丘明明. HPLC法测定病毒清口服液中连翘苷的含量[J]. 中国药房, 2012, 0(15): 1413-1415 |
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| 作者姓名： | 何春玲 丘明明 |
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| 作者单位： | [1]南宁市中医院药剂科,南宁530001 [2]广西食品药品检验所,南宁530021 |
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| 摘 要： | 目的:建立测定病毒清口服液中连翘苷含量的方法。方法:采用高效液相色谱法。色谱柱为Hvydro-RP80AC18(250mm×4.6mm,5μm),流动相为乙腈-水(22:78),流速为1.0mL.min-1,检测波长为277nm,柱温为35℃。结果:连翘苷进样量在0.1005～2.4120μg范围内与峰面积积分值呈良好的线性关系(r=0.9999);平均加样回收率为97.21%,RSD=2.1%(n=9)。结论:本方法操作简便,准确性、重复性好,可用于病毒清口服液中连翘苷的含量测定。
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| 关 键 词： | 病毒清口服液 高效液相色谱法 连翘苷 含量测定 |
Content Determination of Phillyrin in Bingduqing Oral Solution by HPLC |
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| HE Chun-lingNanning Municipal Hospital of Traditional Chinese Medicine,Nanning,China QIU Ming-ming. Content Determination of Phillyrin in Bingduqing Oral Solution by HPLC[J]. China Pharmacy, 2012, 0(15): 1413-1415 |
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| Authors: | HE Chun-lingNanning Municipal Hospital of Traditional Chinese Medicine Nanning China QIU Ming-ming |
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| Affiliation: | HE Chun-ling(Nanning Municipal Hospital of Traditional Chinese Medicine,Nanning 530001,China) QIU Ming-ming(Guangxi Institute for Food and Drug Control,Nanning 530021,China) |
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| Abstract: | OBJECTIVE：To establish the method for the content determination of phillyrin in Bingduqing oral solution.METHODS：HPLC method was used.The Hydro-RP 80A C18（250 mm×4.6 mm,5 μm） column was used with mobile phase consisted of acetonitrile-water（22：78） at the flow rate of 1.0 mL·min-1.The column temperature was 35 ℃ and UV detection was set at 277 nm.RESULTS：The linear range of phillyrin was 0.100 5～2.412 8 μg（r=0.999 9） with an average recovery of 97.21%（RSD=2.1%,n=9）.CONCLUSION：The method is simple,accurate and repeatable.It can be used for the content determination of phillyrin in Bingduqing oral solution. |
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| Keywords: | Bingduqing oral solution HPLC Phillyrin Content determination |
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