RP-HPLC双波长法测定清热解毒软胶囊中栀子苷和黄芩苷的含量

目的:建立同时测定清热解毒软胶囊中栀子苷和黄芩苷含量的方法。方法:采用高效液相色谱法。色谱柱为ZORBAX Eclipse XDB-C18柱;流动相为乙腈(B)-0.2%磷酸水溶液(C)(梯度洗脱,0～9min,15%B～85%C,流速为0.4mL·min-1;10～20min,25%B～75%C,流速为1.0mL·min-1);柱温为30℃,检测波长栀子苷为240nm,黄芩苷为278nm,进样量为10μL。结果:栀子苷、黄芩苷的进样量分别在0.044～0.22μg(r=0.9998)、0.56～2.8μg(r=0.9999)范围内与其峰面积积分值呈良好的线性关系。栀子苷和黄芩苷的低、中、高平均加样回收率分别为98.55%、98.54%、98.56%,98.40%、98.41%、98.42%,RSD分别为0.9197%、0.8323%、0.8596%,0.8653%、0.8058%、0.8693%(n=9)。结论:该方法操作简便、准确度高、重复性和精密度均良好,可用于清热解毒软胶囊质量控制和评价。

Content Determination of Geniposide and Baicalin in Qingre Jiedu Soft Capsules by Dual Wavelength RP-HPLC

OBJECTIVE：To establish a method for the content determination of geniposide and baicalin in Qingre jiedu soft capsules.METHODS：HPLC method was adopted.The determination was performed on ZORBAX Eclipse XDB-C18 column with mobile phase consisted of acetonitrile（B）-0.2% phosphoric acid（C） by gradient elution at the flow rate of 0.4 mL·min-1（0～9 min,15%B～85%C） and 1.0 mL·min-1（10～20 min,25%B～75%C）.The detection wavelength of geniposide and baicalin was set at 240 nm and 278 nm,respectively.The column temperature was maintained at 30 ℃ and injection volume was 10 μL.RESULTS：The linear range of geniposide and baicalin were 0.044～0.22 μg（r=0.999 8） and 0.56～2.8 μg（r=0.999 9）,respectively.Their average recoveries were 98.55%,98.54%,98.56%;98.40%,98.41%,98.42%（RSD=0.919 7%,0.832 3%,0.859 6%;0.865 3%,0.805 8%,0.869 3%）（n=9）,respectively.CONCLUSION：The method is simple,accurate,reproducible and precise,and it is suitable for quality control and evaluation of Qingre jiedu soft capsules.