丁香酚在大鼠体内的组织分布研究

目的:研究丁香酚在大鼠体内的组织分布规律。方法:大鼠ig给予丁香酚后,应用高效液相色谱(HPLC)法测定丁香酚在大鼠心、肝、脾、肺、肾、胃、大肠、小肠、脑、前列腺中的含量。色谱柱为AllsphereODS-2RP-18(250mm×4.6mm,5μm),流动相为甲醇:0.5%冰醋酸=63:37(V/V,pH3.80),检测波长为280nm,柱温为30℃,流速为0.8mL.min-1。结果:丁香酚在大鼠心、肝、脾、肺、肾、胃、大肠、小肠、脑、前列腺的线性回归方程分别为A=185.87c-0.3951(0.9972)、A=174.63c-0.1013(0.9992)、A=164.5c+0.0174(0.9975)、A=191.98c-0.6649(0.9912)、A=192.34c-0.0897(0.9878)、A=180.26c+0.1690(0.9983)、A=146.29c+0.1356(0.9993)、A=159.07c+0.2213(0.9991)、A=158.58c-0.0504(0.9987)、A=97.218c+1.6503(0.9335)。丁香酚组织样品匀浆液浓度在0.011～2.158μg.mL-1范围内与峰面积比值呈良好线性关系。最低检测浓度为0.011μg.mL-1。结论:大鼠ig给药后,较快的分配到各脏器组织中,分布范围广,其中以肠、脾中水平较高,脑中较低。

Study on Tissue Distribution of Eugenol in Rats

OBJECTIVE：To study the tissue distribution regularity of eugenol in rats.METHODS：HPLC method was adopted to determine the contents of eugenol in heart,liver,spleen,lung,kidney,stomach,small intestines,large intestines,brain,prostate,respectively after intragastrical administration of eugenol in rats.Allsphere ODS-2 RP-18 column was used with mobile phase consisted of methanol-0.5%acetic acid（63：37）,（V：V,pH 3.80） at the flow rate of 0.8 mL·min-1.The detection wavelength was set at 280 nm,and column temperature was 30℃.RESULTS：The linear regression equations of eugenol in above tissue were A=185.87c-0.395 1（0.997 2）,A=174.63c-0.101 3（0.999 2）,A=164.5c＋0.017 4（0.9975）,A=191.98c-0.664 9（0.991 2）,A=192.34c-0.089 7（0.987 8）,A=180.26c＋0.169 0（0.998 3）,A=146.29c＋0.135 6（0.999 3）,A=159.07c＋0.221 3（0.999 1）,A=158.58C-0.050 4（0.998 7）,A=97.218c＋1.650 3（0.933 5）,respectively.The linear range of eugenol in homogenate was 0.011～2.158 μg·mL-1 and the lowest detection limut of gentianine was 0.011 μg·mL-1.CONCLUSION：After intragastrical administration,it spread to each viscera and tissue rapidly and widely.The level of it is higher in intestines and spleen,and lower in brain among them.