| RP-HPLC法测定云香十五味丸中栀子苷的含量 |
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| 引用本文： | 陈晓北,邹继红.RP-HPLC法测定云香十五味丸中栀子苷的含量[J].中国药房,2012(7):646-648. |
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| 作者姓名： | 陈晓北 邹继红 |
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| 作者单位： | 赤峰学院医学院,内蒙古赤峰024000 |
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| 摘 要： | 目的:建立测定云香十五味丸中栀子苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Agilent Eclipse XDB-C18(150mm×4.6mm,5μm),流动相为水-乙腈(85:15),流速为1mL.min-1,检测波长为238nm。结果:栀子苷的进样量在0.784～3.920μg范围内与峰面积积分值呈良好线性关系(r=0.9998);平均加样回收率为99.3%,RSD=1.0%(n=9)。结论:本方法简便、快速、准确,适用于云香十五味丸中栀子苷的质量控制。
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| 关 键 词： | 反相高效液相色谱法 云香十五味丸 栀子苷 含量测定 |
Content Determination of Gardenoside in Yunxiang Shiwu Pills by RP-HPLC |
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| CHEN Xiao-bei,ZOU Ji-hong.Content Determination of Gardenoside in Yunxiang Shiwu Pills by RP-HPLC[J].China Pharmacy,2012(7):646-648. |
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| Authors: | CHEN Xiao-bei ZOU Ji-hong |
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| Institution: | (Medical College of Chifeng University,Inner Mongolia Chifeng 024000,China) |
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| Abstract: | OBJECTIVE：To establish the method for the content determination of gardenoside in Yunxiang shiwu pills.METHODS：RP-HPLC method was adopted.The separation was performed on Agilent Eclipse XDB-C18（150 mm×4.6 mm,5 μm） column with mobile phase consisted of water-acetonitrile（85：15） at the flow rate of 1 mL·min-1.The detection wavelength was set at 238 nm.RESULTS：The linear range was 0.784～3.920 μg（r=0.999 8） with an average recovery of 99.3%（RSD=1.0%,n=9）.CONCLUSION：The method is simple,quick,accurate,and is suitable for the quality control of gardenoside in Yunxiang shiwu pills. |
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| Keywords: | RP-HPLC Yunxiang shiwu pills Gardenoside Content determination |
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