CD86在大鼠角膜移植排斥反应中的表达及意义

目的 观察大鼠角膜组织中共刺激分子CD86(B7-2)的原位表达,以了解共刺激分子在大鼠角膜移植排斥反应中的作用和意义。方法 制作大鼠角膜移植模型,观察角膜透明度、新生血管。采用免疫组化法检测CD86在角膜、脾脏组织中的表达。结果 术后角膜植片均出现不同程度的新生血管,角膜水肿、混浊,基质增厚。CD86在正常的角膜组织中无阳性表达;在移植后出现急性排斥反应的角膜上皮层中有大量阳性细胞表达。在脾脏的阳性细胞表达与文献报道的一致。结论 共刺激分子CD86在移植后发生排斥的角膜组织中的阳性表达,可能与免疫排斥反应有关。

Immunohistochemical study of in situ CD86 expression in rejected rat corneal graft after penetrating keratoplasty

Objective To investigate the expression of CD86 co-stimulatory molecules in rejected rat corneal graft in situ after penetrating keratoplasty. Methods Rat models of orthotopical corneal transplantation were established, and after the occurrence of graft rejection, immunohistochemical staining was performed on the corneal whole-mounts and the spleen tissue of the recipient rats. Results CD86 cells were detected in the epithelium of the corneal graft during allograft rejection but not in normal cornea. Conclusion In situ expression of costimulatory molecules in the corneal allograft may be related to the acute immune rejection after penetrating keratoplasty.