活血药对血管紧张素Ⅱ致心肌肥大细胞内钙浓度变化的影响

目的研究活血药丹参素和川芎嗪对血管紧张素Ⅱ诱导的乳鼠心肌细胞内和心肌成纤维细胞内钙离子浓度的影响，探讨其抑制心肌肥大的作用机理。方法应用Fura-3荧光染料，用流式细胞仪检测乳鼠心肌细胞和心肌成纤维细胞内钙离子浓度。结果AngⅡ可明显增加心肌细胞和心肌成纤维细胞内钙离子浓度，并随着时间的延长，于第5天其浓度达到高峰，然后下降，洛沙坦（Losartan）明显抑制其心肌和心肌成纤维内钙离子浓度的增加；研究显示丹参素和川芎嗪不仅明显抑制血管紧张素Ⅱ诱导心肌细胞内钙离子浓度的升高，同时也明显抑制血管紧张素Ⅱ诱导心肌成纤维细胞内钙离子浓度的升高。结论丹参素和川芎嗪不仅明显抑制血管紧张素Ⅱ诱导心肌细胞内钙离子浓度的升高，同时也明显抑制血管紧张素Ⅱ诱导心肌成纤维细胞内钙离子浓度的升高，表明丹参素和川芎嗪可能具有钙拮抗剂的作用，抑制血管紧张素Ⅱ所介导引起细胞内钙离子升高，从而抑制心肌细胞肥大和心肌成纤维细胞增殖。

Influence of drugs for promoting blood circulation on Ca2+ in myocardial cells and myocardial fibroblast induced by angiotensin Ⅱ

Objective To study the effective mechanism of Danshensu and Lignstrazine, the effective fractions in Chinese herbal medicines with drugs for promoting blood circulation, on Ca^2＋ in neonatal mice＇ s myocardial cells and myocardial fibroblast induced by angiotensinⅡ. Methods Ca^2＋ in myocardial cells and myocardial fibroblast has been detected by cooher EPICS XL with Fura-3. Results Not only can the Danshensu and Ligustrazine inhibit the increase of Ca^2＋ concentration of myocardial cells significantly, but also the proliferation of myocardial fibroblasts. The mechanisms may be Danshensu and Lignstrazine as Ca^2＋ antagonists, which reduce the increase of Ca^2＋ concentration induced by AngⅡ. Conclusion It showed that the drugs for promoting blood circulation decrease the high Ca^2＋ concentration induced by Ang Ⅱ, so that they can inhibit hypertrophy of cardiac myocytes and hyperplasia of cardiac fibroblasts and prevent LVH.